TY - JOUR
T1 - Spontaneous and induced differentiation of human melanoma cells
AU - Valyi‐Nagy, Istvan
AU - Shih, Ie‐Ming ‐M
AU - Györfi, Tibor
AU - Greenstein, David
AU - Juhasz, Istvan
AU - Herlyn, Meenhard
AU - Elder, David E.
PY - 1993/4/22
Y1 - 1993/4/22
N2 - Malignant melanoma cells can differentiate spontaneously in vivo and in vitro into cells with a finite lifespan. Analysis of differentiating cells from primary melanomas in culture revealed a flat, fibroblast‐like morphology and expression of the fibroblast‐associated marker leucine aminopeptidase (LAP). Differentiation was also observed in a minor sub‐population of permanent cell lines derived from metastatic lesions. An experimental model of melanoma cell differentiation was then developed, using the pyrimidine analog bromodeoxyuridine (BUdR). BUdR‐treated cells had a flat morphology, were contact‐inhibited, had up to 20‐fold increased surface area, expressed LAP, no longer proliferated anchorage‐independently in soft agar, and 3 out of 4 cell lines were non‐tumorigenic in athymic nude mice. Our results show that models of differentiation of melanoma cells can be established that help to define pathways of differentiation.
AB - Malignant melanoma cells can differentiate spontaneously in vivo and in vitro into cells with a finite lifespan. Analysis of differentiating cells from primary melanomas in culture revealed a flat, fibroblast‐like morphology and expression of the fibroblast‐associated marker leucine aminopeptidase (LAP). Differentiation was also observed in a minor sub‐population of permanent cell lines derived from metastatic lesions. An experimental model of melanoma cell differentiation was then developed, using the pyrimidine analog bromodeoxyuridine (BUdR). BUdR‐treated cells had a flat morphology, were contact‐inhibited, had up to 20‐fold increased surface area, expressed LAP, no longer proliferated anchorage‐independently in soft agar, and 3 out of 4 cell lines were non‐tumorigenic in athymic nude mice. Our results show that models of differentiation of melanoma cells can be established that help to define pathways of differentiation.
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U2 - 10.1002/ijc.2910540125
DO - 10.1002/ijc.2910540125
M3 - Article
C2 - 8478142
AN - SCOPUS:0027238879
SN - 0020-7136
VL - 54
SP - 159
EP - 165
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 1
ER -