Splenic imaging with 99Tc(m) labeled erythrocytes: A comparative study of cell damaging methods

R. G. Hamilton, P. O. Alderson, J. F. Harwig, B. A. Siegel

Research output: Contribution to journalArticlepeer-review


Several methods of damaging red blood cells (RBCs) for splenic imaging were compared to determine the optimum approach. The RBCs from donor animals were labeled with (99m)TcO4- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N ethylmaleimide (NEM) or p hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBCs were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen to liver ratios with heat or sulfhydryl damaged (99m)Tc RBCs were significantly greater (p <0.001) than the values obtained using ACD or Sn(II) ion. Heat damaging produces good splenic localization of (99m)Tc RBCs but requires rigidly controlled incubation conditions. NEM damaging provides an excellent and predictable alternative approach.

Original languageEnglish (US)
Pages (from-to)1038-1043
Number of pages6
JournalJournal of Nuclear Medicine
Issue number12
StatePublished - Jan 1 1976
Externally publishedYes

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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