Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus

Carmen Butan, Lisa M. Hartnell, Andrew K. Fenton, Donald Frank Bliss, R. Elizabeth Sockett, Sriram Subramaniam, Jacqueline L S Milne

Research output: Contribution to journalArticle

Abstract

We present a cryo-electron tomographic analysis of the three-dimensional architecture of a strain of the Gram-negative bacterium Bdellovibrio bacteriovorus in which endogenous MreB2 was replaced with monomeric teal fluorescent protein (mTFP)-labeled MreB2. In contrast to wild-type Bdellovibrio cells that predominantly displayed a compact nucleoid region, cells expressing mTFP-labeled MreB2 displayed a twisted spiral organization of the nucleoid. The more open structure of the MreB2-mTFP nucleoids enabled clear in situ visualization of ribosomes decorating the periphery of the nucleoid. Ribosomes also bordered the edges of more compact nucleoids from both wild-type cells and mutant cells. Surprisingly, MreB2-mTFP localized to the interface between the spiral nucleoid and the cytoplasm, suggesting an intimate connection between nucleoid architecture and MreB arrangement. Further, in contrast to wild-type cells, where a single tight chemoreceptor cluster localizes close to the single polar flagellum, MreB2-mTFP cells often displayed extended chemoreceptor arrays present at one or both poles and displayed multiple or inaccurately positioned flagella. Our findings provide direct structural evidence for spiral organization of the bacterial nucleoid and suggest a possible role for MreB in regulation of nucleoid architecture and localization of the chemotaxis apparatus.

Original languageEnglish (US)
Pages (from-to)1341-1350
Number of pages10
JournalJournal of Bacteriology
Volume193
Issue number6
DOIs
StatePublished - Mar 2011
Externally publishedYes

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Flagella
Ribosomes
Bdellovibrio
Chemotaxis
Gram-Negative Bacteria
Bdellovibrio bacteriovorus
Cytoplasm
Cyan Fluorescent Protein
Electrons

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Butan, C., Hartnell, L. M., Fenton, A. K., Bliss, D. F., Sockett, R. E., Subramaniam, S., & Milne, J. L. S. (2011). Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus. Journal of Bacteriology, 193(6), 1341-1350. https://doi.org/10.1128/JB.01061-10

Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus. / Butan, Carmen; Hartnell, Lisa M.; Fenton, Andrew K.; Bliss, Donald Frank; Sockett, R. Elizabeth; Subramaniam, Sriram; Milne, Jacqueline L S.

In: Journal of Bacteriology, Vol. 193, No. 6, 03.2011, p. 1341-1350.

Research output: Contribution to journalArticle

Butan, C, Hartnell, LM, Fenton, AK, Bliss, DF, Sockett, RE, Subramaniam, S & Milne, JLS 2011, 'Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus', Journal of Bacteriology, vol. 193, no. 6, pp. 1341-1350. https://doi.org/10.1128/JB.01061-10
Butan C, Hartnell LM, Fenton AK, Bliss DF, Sockett RE, Subramaniam S et al. Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus. Journal of Bacteriology. 2011 Mar;193(6):1341-1350. https://doi.org/10.1128/JB.01061-10
Butan, Carmen ; Hartnell, Lisa M. ; Fenton, Andrew K. ; Bliss, Donald Frank ; Sockett, R. Elizabeth ; Subramaniam, Sriram ; Milne, Jacqueline L S. / Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus. In: Journal of Bacteriology. 2011 ; Vol. 193, No. 6. pp. 1341-1350.
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