Sphingosine-1-phosphate regulates the expression of adherens junction protein E-cadherin and enhances intestinal epithelial cell barrier function

Jose Greenspon, Ruiyun Li, Lan Xiao, Jaladanki N. Rao, Rex Sun, Eric D. Strauch, Terez Shea-Donohue, Jian Ying Wang, Douglas J. Turner

Research output: Contribution to journalArticle

Abstract

Background: The regulation of intestinal barrier permeability is important in the maintenance of normal intestinal physiology. Sphingosine-1-phosphate (S1P) has been shown to play a pivotal role in enhancing barrier function in several non-intestinal tissues. The current study determined whether S1P regulated function of the intestinal epithelial barrier by altering expression of E-cadherin, an important protein in adherens junctions. Methods: Studies were performed upon cultured differentiated IECs (IEC-Cdx2L1 line) using standard techniques. Results: S1P treatment significantly increased levels of E-cadherin protein and mRNA in intestinal epithelial cells (IECs) and also led to E-cadherin localizing strongly to the cell-cell border. S1P also improved the barrier function as indicated by a decrease in 14C-mannitol paracellular permeability and an increase in transepithelial electrical resistance (TEER) in vitro. Conclusions: These results indicate that S1P increases levels of E-cadherin, both in cellular amounts and at the cell-cell junctions, and leads to improved barrier integrity in cultured intestinal epithelial cells.

Original languageEnglish (US)
Pages (from-to)1342-1353
Number of pages12
JournalDigestive diseases and sciences
Volume56
Issue number5
DOIs
StatePublished - May 2011

Keywords

  • Ca signaling
  • Intestinal barrier
  • Intestinal epithelium
  • Sphingosine

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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  • Cite this

    Greenspon, J., Li, R., Xiao, L., Rao, J. N., Sun, R., Strauch, E. D., Shea-Donohue, T., Wang, J. Y., & Turner, D. J. (2011). Sphingosine-1-phosphate regulates the expression of adherens junction protein E-cadherin and enhances intestinal epithelial cell barrier function. Digestive diseases and sciences, 56(5), 1342-1353. https://doi.org/10.1007/s10620-010-1421-0