Sphingomyelinase activity in human platelets

Carl G. Simon, Subroto B Chatterjee, Adrian R L Gear

Research output: Contribution to journalArticle

Abstract

The sphingolipid metabolites, ceramide, sphingosine, and sphingosine-1- phosphate, may be involved in several signalling pathways and may regulate cell functions such as cell growth, secretion, differentiation, and apoptosis. During activation of human platelets by thrombin, sphingosine-1- phosphate is released from platelets and can potentiate their aggregation. Thrombin also causes an increase in platelet sphingosine levels. Since these molecules can be derived from sphingomyelin, we have determined whether platelets possess sphingomyelinase and whether this enzyme is regulated during platelet function. Using radioactive sphingomyelin as substrate, we assayed sphingomyelinase activity over the range of pH 4 to 10 and observed optimal activity at pH 5.0-5.5. Little activity was found at neutral or alkaline pH, and the presence of Mg++, Ca++, Zn++, or EDTA in the reaction mixture had little effect on the pH profile. Activation of platelets by thrombin or ADP had no effect on sphingomyelinase activity, but thrombin caused secretion of the acid-sphingomyelinase activity into the media. Thus, human platelets contain an acid-sphingomyelinase which is secreted during thrombin-induced platelet activation.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalThrombosis Research
Volume90
Issue number4
DOIs
StatePublished - May 15 1998

Fingerprint

Sphingomyelin Phosphodiesterase
Human Activities
Thrombin
Blood Platelets
Platelet Activation
Sphingosine
Sphingomyelins
Sphingolipids
Acids
Ceramides
Edetic Acid
Adenosine Diphosphate
Apoptosis
Enzymes
Growth

Keywords

  • Platelets
  • Secretion
  • Sphingomyelinase

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Sphingomyelinase activity in human platelets. / Simon, Carl G.; Chatterjee, Subroto B; Gear, Adrian R L.

In: Thrombosis Research, Vol. 90, No. 4, 15.05.1998, p. 155-161.

Research output: Contribution to journalArticle

Simon, Carl G. ; Chatterjee, Subroto B ; Gear, Adrian R L. / Sphingomyelinase activity in human platelets. In: Thrombosis Research. 1998 ; Vol. 90, No. 4. pp. 155-161.
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