Spermidine labels proteins during sea urchin embryogenesis

Z. N. Canellakis, L. L. Marsh, Y. C. Manabe, A. A. Infante, P. K. Bondy, F. W. Scalise

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously described the presence of a protein containing intact, covalently bound spermidine during very early embryogenesis of the sea urchin (Strongylocentrotus purpuratus). Proteins containing other polyamine metabolites also appear as embryogenesis proceeds. These proteins which contain label derived from exogenous radioactive spermidine show a characteristic pattern which changes during the course of embryonic development. We document for the first time that hypusine, the polyamine metabolite which is a characteristic component of the eukaryotic protein translation initiation factor eIF-4D, is present in more than one species of macromolecule. In addition, N1-acetylspermidine has also been identified as a significant intracellular metabolite of spermidine during embryogenesis.

Original languageEnglish (US)
Pages (from-to)969-976
Number of pages8
JournalBiochemistry International
Volume19
Issue number5
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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