Spermidine labels proteins during sea urchin embryogenesis

Z. N. Canellakis, L. L. Marsh, Yukari C Manabe, A. A. Infante, P. K. Bondy, F. W. Scalise

Research output: Contribution to journalArticle

Abstract

We have previously described the presence of a protein containing intact, covalently bound spermidine during very early embryogenesis of the sea urchin (Strongylocentrotus purpuratus). Proteins containing other polyamine metabolites also appear as embryogenesis proceeds. These proteins which contain label derived from exogenous radioactive spermidine show a characteristic pattern which changes during the course of embryonic development. We document for the first time that hypusine, the polyamine metabolite which is a characteristic component of the eukaryotic protein translation initiation factor eIF-4D, is present in more than one species of macromolecule. In addition, N1-acetylspermidine has also been identified as a significant intracellular metabolite of spermidine during embryogenesis.

Original languageEnglish (US)
Pages (from-to)969-976
Number of pages8
JournalBiochemistry International
Volume19
Issue number5
StatePublished - 1989
Externally publishedYes

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Spermidine
Sea Urchins
Embryonic Development
Labels
Metabolites
Polyamines
Proteins
Translational Peptide Chain Initiation
Strongylocentrotus purpuratus
Eukaryotic Initiation Factors
Peptide Initiation Factors
Macromolecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Canellakis, Z. N., Marsh, L. L., Manabe, Y. C., Infante, A. A., Bondy, P. K., & Scalise, F. W. (1989). Spermidine labels proteins during sea urchin embryogenesis. Biochemistry International, 19(5), 969-976.

Spermidine labels proteins during sea urchin embryogenesis. / Canellakis, Z. N.; Marsh, L. L.; Manabe, Yukari C; Infante, A. A.; Bondy, P. K.; Scalise, F. W.

In: Biochemistry International, Vol. 19, No. 5, 1989, p. 969-976.

Research output: Contribution to journalArticle

Canellakis, ZN, Marsh, LL, Manabe, YC, Infante, AA, Bondy, PK & Scalise, FW 1989, 'Spermidine labels proteins during sea urchin embryogenesis', Biochemistry International, vol. 19, no. 5, pp. 969-976.
Canellakis ZN, Marsh LL, Manabe YC, Infante AA, Bondy PK, Scalise FW. Spermidine labels proteins during sea urchin embryogenesis. Biochemistry International. 1989;19(5):969-976.
Canellakis, Z. N. ; Marsh, L. L. ; Manabe, Yukari C ; Infante, A. A. ; Bondy, P. K. ; Scalise, F. W. / Spermidine labels proteins during sea urchin embryogenesis. In: Biochemistry International. 1989 ; Vol. 19, No. 5. pp. 969-976.
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