Spermatogenesis in vitro: completion of meiosis and early spermiogenesis.

M. Parvinen, William W Wright, D. M. Phillips, J. P. Mather, N. A. Musto, C. W. Bardin

Research output: Contribution to journalArticle

Abstract

In vitro formation of haploid spermatids has not been convincingly demonstrated in mammals. To investigate this problem we selected defined segments of rat seminiferous tubules containing late pachytene and diakinetic primary spermatocytes (Stages XII and XIII of the cycle) for culture in a chemically defined medium. After 2 days, most spermatocytes completed both meiotic divisions, and by 6 days the tubular epithelium developed morphologic characteristics of Stage V in which the newly formed spermatids had acrosomic systems characteristic of step 5 spermiogenesis. The seminiferous tubules also differentiated biochemically as evidenced by increased production of proteins characteristically secreted by Stage V. Since this in vitro differentiation of the germinal epithelium occurred in the absence of testosterone and FSH, we conclude that late pachytene spermatocytes and their associated Sertoli cells have all the information required for both meiotic divisions and early spermiogenesis.

Original languageEnglish (US)
Pages (from-to)1150-1152
Number of pages3
JournalEndocrinology
Volume112
Issue number3
StatePublished - Mar 1983
Externally publishedYes

Fingerprint

Spermatocytes
Meiosis
Spermatogenesis
Seminiferous Tubules
Spermatids
Epithelium
Sertoli Cells
Haploidy
Testosterone
Mammals
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Parvinen, M., Wright, W. W., Phillips, D. M., Mather, J. P., Musto, N. A., & Bardin, C. W. (1983). Spermatogenesis in vitro: completion of meiosis and early spermiogenesis. Endocrinology, 112(3), 1150-1152.

Spermatogenesis in vitro : completion of meiosis and early spermiogenesis. / Parvinen, M.; Wright, William W; Phillips, D. M.; Mather, J. P.; Musto, N. A.; Bardin, C. W.

In: Endocrinology, Vol. 112, No. 3, 03.1983, p. 1150-1152.

Research output: Contribution to journalArticle

Parvinen, M, Wright, WW, Phillips, DM, Mather, JP, Musto, NA & Bardin, CW 1983, 'Spermatogenesis in vitro: completion of meiosis and early spermiogenesis.', Endocrinology, vol. 112, no. 3, pp. 1150-1152.
Parvinen M, Wright WW, Phillips DM, Mather JP, Musto NA, Bardin CW. Spermatogenesis in vitro: completion of meiosis and early spermiogenesis. Endocrinology. 1983 Mar;112(3):1150-1152.
Parvinen, M. ; Wright, William W ; Phillips, D. M. ; Mather, J. P. ; Musto, N. A. ; Bardin, C. W. / Spermatogenesis in vitro : completion of meiosis and early spermiogenesis. In: Endocrinology. 1983 ; Vol. 112, No. 3. pp. 1150-1152.
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