Abstract
We report on novel hyper-spectral imaging filter-modules based on acousto-optic tuneable filters (AOTF). The AOTF functions as a full-field tuneable bandpass filter which offers fast continuous or random access tuning with high filtering efficiency. Due to the diffractive nature of the device, the unfiltered zero-order and the filtered first-order images are geometrically separated. The modules developed exploit this feature to simultaneously route both the transmitted white-light image and the filtered fluorescence image to two separate cameras. Incorporation of prisms in the optical paths and careful design of the relay optics in the filter module have overcome a number of aberrations inherent to imaging through AOTFs, leading to excellent spatial resolution. A number of practical uses of this technique, both for in vivo auto-fluorescence endoscopy and in vitro fluorescence microscopy were demonstrated. We describe the operational principle and design of recently improved prototype instruments for fluorescence-based diagnostics and demonstrate their performance by presenting challenging hyper-spectral fluorescence imaging applications.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6628 |
| State | Published - 2007 |
| Externally published | Yes |
| Event | Diagnostic Optical Spectroscopy in Biomedicine IV - Munich, Germany Duration: Jun 19 2007 → Jun 21 2007 |
Other
| Other | Diagnostic Optical Spectroscopy in Biomedicine IV |
|---|---|
| Country | Germany |
| City | Munich |
| Period | 6/19/07 → 6/21/07 |
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Keywords
- AOTF
- Autofluorescence
- Endoscopy
- Microscopy
- Spectroscopy
ASJC Scopus subject areas
- Engineering(all)
Cite this
Spectroscopic imaging using acousto-optic tuneable filters. / Bouhifd, Mounir; Whelan, Maurice.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6628 2007. 662803.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Spectroscopic imaging using acousto-optic tuneable filters
AU - Bouhifd, Mounir
AU - Whelan, Maurice
PY - 2007
Y1 - 2007
N2 - We report on novel hyper-spectral imaging filter-modules based on acousto-optic tuneable filters (AOTF). The AOTF functions as a full-field tuneable bandpass filter which offers fast continuous or random access tuning with high filtering efficiency. Due to the diffractive nature of the device, the unfiltered zero-order and the filtered first-order images are geometrically separated. The modules developed exploit this feature to simultaneously route both the transmitted white-light image and the filtered fluorescence image to two separate cameras. Incorporation of prisms in the optical paths and careful design of the relay optics in the filter module have overcome a number of aberrations inherent to imaging through AOTFs, leading to excellent spatial resolution. A number of practical uses of this technique, both for in vivo auto-fluorescence endoscopy and in vitro fluorescence microscopy were demonstrated. We describe the operational principle and design of recently improved prototype instruments for fluorescence-based diagnostics and demonstrate their performance by presenting challenging hyper-spectral fluorescence imaging applications.
AB - We report on novel hyper-spectral imaging filter-modules based on acousto-optic tuneable filters (AOTF). The AOTF functions as a full-field tuneable bandpass filter which offers fast continuous or random access tuning with high filtering efficiency. Due to the diffractive nature of the device, the unfiltered zero-order and the filtered first-order images are geometrically separated. The modules developed exploit this feature to simultaneously route both the transmitted white-light image and the filtered fluorescence image to two separate cameras. Incorporation of prisms in the optical paths and careful design of the relay optics in the filter module have overcome a number of aberrations inherent to imaging through AOTFs, leading to excellent spatial resolution. A number of practical uses of this technique, both for in vivo auto-fluorescence endoscopy and in vitro fluorescence microscopy were demonstrated. We describe the operational principle and design of recently improved prototype instruments for fluorescence-based diagnostics and demonstrate their performance by presenting challenging hyper-spectral fluorescence imaging applications.
KW - AOTF
KW - Autofluorescence
KW - Endoscopy
KW - Microscopy
KW - Spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=36249020390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36249020390&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:36249020390
SN - 0819467723
SN - 9780819467720
VL - 6628
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -