Spectroscopic imaging using acousto-optic tuneable filters

Mounir Bouhifd, Maurice Whelan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


We report on novel hyper-spectral imaging filter-modules based on acousto-optic tuneable filters (AOTF). The AOTF functions as a full-field tuneable bandpass filter which offers fast continuous or random access tuning with high filtering efficiency. Due to the diffractive nature of the device, the unfiltered zero-order and the filtered first-order images are geometrically separated. The modules developed exploit this feature to simultaneously route both the transmitted white-light image and the filtered fluorescence image to two separate cameras. Incorporation of prisms in the optical paths and careful design of the relay optics in the filter module have overcome a number of aberrations inherent to imaging through AOTFs, leading to excellent spatial resolution. A number of practical uses of this technique, both for in vivo auto-fluorescence endoscopy and in vitro fluorescence microscopy were demonstrated. We describe the operational principle and design of recently improved prototype instruments for fluorescence-based diagnostics and demonstrate their performance by presenting challenging hyper-spectral fluorescence imaging applications.

Original languageEnglish (US)
Title of host publicationDiagnostic Optical Spectroscopy in Biomedicine IV
ISBN (Print)0819467723, 9780819467720
StatePublished - 2007
Externally publishedYes
EventDiagnostic Optical Spectroscopy in Biomedicine IV - Munich, Germany
Duration: Jun 19 2007Jun 21 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherDiagnostic Optical Spectroscopy in Biomedicine IV


  • AOTF
  • Autofluorescence
  • Endoscopy
  • Microscopy
  • Spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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