SPECT measurement of benzodiazepine receptors in human brain with iodine- 123-iomazenil: Kinetic and equilibrium paradigms

A. Abi-Dargham, M. Laruelle, J. Seibyl, Z. Rattner, R. M. Baldwin, S. S. Zoghbi, Y. Zea-Ponce, J. D. Bremner, T. M. Hyde, D. S. Charney, P. B. Hoffer, R. B. Innis

Research output: Contribution to journalArticlepeer-review

Abstract

Iodine-123-iomazenil binding to benzodiazepine receptors in human brain was measured with SPECT using kinetic and equilibrium methods. Methods: In the kinetic experiments (n = 6), regional time-activity curves after a single bolus injection of the tracer were fit to a three-compartment model to provide estimates of the rate constants K1 to k4. The binding potential (equal to the product of the receptor density and affinity) was derived from the rate constants. In the equilibrium method (n = 8), the tracer bolus injection was followed by a constant tracer infusion to induce a sustained equilibrium state. The regional equilibrium volume of distribution was calculated as the ratio of the regional brain concentration-to-the free parent tracer steady-state plasma concentration. In three experiments, a receptor-saturating dose of flumazenil was injected for direct measurement of the nondisplaceable compartment distribution volume. Results: The kinetic and equilibrium method results were in good agreement in all regions investigated. Iodine-125-iomazenil binding potential measured in vitro in 12 postmortem samples was found to be consistent with SPECT in vivo measurements. Conclusion: These studies demonstrated the feasibility of quantification of receptor binding with SPECT.

Original languageEnglish (US)
Pages (from-to)228-238
Number of pages11
JournalJournal of Nuclear Medicine
Volume35
Issue number2
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • SPECT
  • benzodiazepine receptors
  • brain
  • iodine-123-iomazenil

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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