Specimen dilution improves sensitivity of the amplified Mycobacterium tuberculosis direct test for smear microscopy-positive respiratory specimens

Renata L. Guerra, James F. Baker, Roya Alborz, Derek T. Armstrong, Julia A. Kiehlbauch, Marcus B. Conde, Susan E. Dorman, Nancy M. Hooper

Research output: Contribution to journalArticle

Abstract

Specimen dilution has been proposed as a strategy to minimize amplified Mycobacterium tuberculosis direct (MTD) test inhibition (N. Pollock, J. Westerling, and A. Sloutsky, Am. J. Clin. Pathol. 126:142-147, 2006; A. Sloutsky, L. L. Han, and B. G. Werner, J. Clin. Microbiol. 42:1547-1551, 2004). We evaluated the impact of respiratory specimen dilution on MTD test accuracy in a public health laboratory. The difference in MTD test sensitivity between the dilution and conventional methods was 15.9% (P = 0.001) for smear microscopy-positive specimens and -3.6% (P = 0.38) for smear microscopy-negative specimens.

Original languageEnglish (US)
Pages (from-to)314-316
Number of pages3
JournalJournal of Clinical Microbiology
Volume46
Issue number1
DOIs
StatePublished - Jan 2008

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Mycobacterium tuberculosis
Microscopy
Public Health

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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Specimen dilution improves sensitivity of the amplified Mycobacterium tuberculosis direct test for smear microscopy-positive respiratory specimens. / Guerra, Renata L.; Baker, James F.; Alborz, Roya; Armstrong, Derek T.; Kiehlbauch, Julia A.; Conde, Marcus B.; Dorman, Susan E.; Hooper, Nancy M.

In: Journal of Clinical Microbiology, Vol. 46, No. 1, 01.2008, p. 314-316.

Research output: Contribution to journalArticle

Guerra, Renata L. ; Baker, James F. ; Alborz, Roya ; Armstrong, Derek T. ; Kiehlbauch, Julia A. ; Conde, Marcus B. ; Dorman, Susan E. ; Hooper, Nancy M. / Specimen dilution improves sensitivity of the amplified Mycobacterium tuberculosis direct test for smear microscopy-positive respiratory specimens. In: Journal of Clinical Microbiology. 2008 ; Vol. 46, No. 1. pp. 314-316.
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