Specimen dilution improves sensitivity of the amplified Mycobacterium tuberculosis direct test for smear microscopy-positive respiratory specimens

Renata L. Guerra, James F. Baker, Roya Alborz, Derek T. Armstrong, Julia A. Kiehlbauch, Marcus B. Conde, Susan E. Dorman, Nancy M. Hooper

Research output: Contribution to journalArticle

Abstract

Specimen dilution has been proposed as a strategy to minimize amplified Mycobacterium tuberculosis direct (MTD) test inhibition (N. Pollock, J. Westerling, and A. Sloutsky, Am. J. Clin. Pathol. 126:142-147, 2006; A. Sloutsky, L. L. Han, and B. G. Werner, J. Clin. Microbiol. 42:1547-1551, 2004). We evaluated the impact of respiratory specimen dilution on MTD test accuracy in a public health laboratory. The difference in MTD test sensitivity between the dilution and conventional methods was 15.9% (P = 0.001) for smear microscopy-positive specimens and -3.6% (P = 0.38) for smear microscopy-negative specimens.

Original languageEnglish (US)
Pages (from-to)314-316
Number of pages3
JournalJournal of clinical microbiology
Volume46
Issue number1
DOIs
StatePublished - Jan 2008

ASJC Scopus subject areas

  • Microbiology (medical)

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    Guerra, R. L., Baker, J. F., Alborz, R., Armstrong, D. T., Kiehlbauch, J. A., Conde, M. B., Dorman, S. E., & Hooper, N. M. (2008). Specimen dilution improves sensitivity of the amplified Mycobacterium tuberculosis direct test for smear microscopy-positive respiratory specimens. Journal of clinical microbiology, 46(1), 314-316. https://doi.org/10.1128/JCM.01313-07