Specificity of G protein β and γ subunit interactions

C. J. Schmidt, T. C. Thomas, M. A. Levine, E. J. Neer

    Research output: Contribution to journalArticle

    Abstract

    Multiple heterotrimeric guanine nucleotide binding protein (G protein) subunits have evolved to couple a large variety of receptors to intracellular effectors. G protein βγ subunits are essential for efficient coupling of α subunits to receptors, and they are also important for modulation of effectors. Several different β and γ subunits exist, but it is not known whether all possible combinations of β and γ can form functional dimers. To answer this question, we have compared the ability of in vitro translated β1, β2, and β3 to form dimers with either γ1 or γ2. Dimerization was monitored by gel filtration, resistance to tryptic digestion, and chemical cross-linking. The results indicate that β1 binds both γ subunits, β2 binds only γ2, and β3 will bind neither γ1 or γ2. Hence, the occurrence of βγ dimers may be partially regulated by the ability of the subunits to associate. Specificity of dimerization might allow cells to co-express multiple β and γ subunits while maintaining efficient and specific signal transduction.

    Original languageEnglish (US)
    Pages (from-to)13807-13810
    Number of pages4
    JournalJournal of Biological Chemistry
    Volume267
    Issue number20
    StatePublished - 1992

    Fingerprint

    Protein Subunits
    Dimerization
    GTP-Binding Proteins
    Dimers
    Guanine Nucleotides
    Gel Chromatography
    Digestion
    Signal Transduction
    Signal transduction
    Carrier Proteins
    Gels
    Modulation
    In Vitro Techniques

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Schmidt, C. J., Thomas, T. C., Levine, M. A., & Neer, E. J. (1992). Specificity of G protein β and γ subunit interactions. Journal of Biological Chemistry, 267(20), 13807-13810.

    Specificity of G protein β and γ subunit interactions. / Schmidt, C. J.; Thomas, T. C.; Levine, M. A.; Neer, E. J.

    In: Journal of Biological Chemistry, Vol. 267, No. 20, 1992, p. 13807-13810.

    Research output: Contribution to journalArticle

    Schmidt, CJ, Thomas, TC, Levine, MA & Neer, EJ 1992, 'Specificity of G protein β and γ subunit interactions', Journal of Biological Chemistry, vol. 267, no. 20, pp. 13807-13810.
    Schmidt CJ, Thomas TC, Levine MA, Neer EJ. Specificity of G protein β and γ subunit interactions. Journal of Biological Chemistry. 1992;267(20):13807-13810.
    Schmidt, C. J. ; Thomas, T. C. ; Levine, M. A. ; Neer, E. J. / Specificity of G protein β and γ subunit interactions. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 20. pp. 13807-13810.
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