Specificity and modes of the anion exchanger in dog renal microvillus membranes

S. E. Guggino, G. J. Martin, P. S. Aronson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The transport of various organic anions via the pathway that mediates the exchange of urate or p-aminohippurate (PAH) for OH- or Cl- in dog renal microvillus membrane vesicles was investigated. The pH gradient-stimulated uptakes of tracer urate and PAH were significantly inhibited by 5 mM PAH, n-valerate, lactate, β-hydroxybutyrate, oxaloacetate, and cis-aconitate but not by 5 mM acetate, malate, oxalate, or citrate. The pH dependence of inhibition suggested that it was in their monovalent forms that these acid anions interacted with the urate exchange pathway. Outwardly directed gradients of succinate, lactate, and PAH stimulated uphill urate accumulation. Imposition of an inside-alkaline pH gradient stimulated the uphill accumulation of lactate and succinate. Na+ cotransport pathways for lactate and succinate were also present. In the presence of an inwardly directed Na+ gradient, lactate stimulated the uphill accumulation of urate, indicating that the pathways mediating Na+-lactate cotransport and lactate-urate exchange coexisted in at least some membrane vesicles. We conclude that the anion exchange pathway for urate in dog renal microvillus membrane vesicles has affinity for additional organic anions and can function in multiple exchange modes. Exchange of luminal urate or Cl- for intracellular organic anions or OH- is a possible mechanism for effecting uphill anion reabsorption in the proximal tubule.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume13
Edition6
StatePublished - 1983
Externally publishedYes

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Microvilli
Uric Acid
Anions
Lactic Acid
Dogs
p-Aminohippuric Acid
Kidney
Membranes
Succinic Acid
Proton-Motive Force
Aconitic Acid
Hydroxybutyrates
Valerates
Oxaloacetic Acid
Oxalates
Citric Acid
Acetates
Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Guggino, S. E., Martin, G. J., & Aronson, P. S. (1983). Specificity and modes of the anion exchanger in dog renal microvillus membranes. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (6 ed., Vol. 13)

Specificity and modes of the anion exchanger in dog renal microvillus membranes. / Guggino, S. E.; Martin, G. J.; Aronson, P. S.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 13 6. ed. 1983.

Research output: Chapter in Book/Report/Conference proceedingChapter

Guggino, SE, Martin, GJ & Aronson, PS 1983, Specificity and modes of the anion exchanger in dog renal microvillus membranes. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 6 edn, vol. 13.
Guggino SE, Martin GJ, Aronson PS. Specificity and modes of the anion exchanger in dog renal microvillus membranes. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 6 ed. Vol. 13. 1983
Guggino, S. E. ; Martin, G. J. ; Aronson, P. S. / Specificity and modes of the anion exchanger in dog renal microvillus membranes. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 13 6. ed. 1983.
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