Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles

Jeff W.M. Bulte, Yke Hoekstra, Richard L. Kamman, Richard L. Magin, Andrew G. Webb, Richard W. Briggs, K. Gwan Go, Caesar E. Hulstaert, Stefan Miltenyi, T. Hauw The, Lou De Leij

Research output: Contribution to journalArticle

Abstract

Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.

Original languageEnglish (US)
Pages (from-to)148-157
Number of pages10
JournalMagnetic resonance in medicine
Volume25
Issue number1
DOIs
StatePublished - May 1992

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Bulte, J. W. M., Hoekstra, Y., Kamman, R. L., Magin, R. L., Webb, A. G., Briggs, R. W., Gwan Go, K., Hulstaert, C. E., Miltenyi, S., Hauw The, T., & De Leij, L. (1992). Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles. Magnetic resonance in medicine, 25(1), 148-157. https://doi.org/10.1002/mrm.1910250115