Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles

Jeff W Bulte, Y. Hoekstra, R. L. Kamman, R. L. Magin, A. G. Webb, R. W. Briggs, K. G. Go, C. E. Hulstaert, S. Miltenyi, T. H. The, L. De Leij

Research output: Contribution to journalArticle

Abstract

Human lymphocytes were labeled with biotinylated anti-lymphocyte-directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran-magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R1. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.

Original languageEnglish (US)
Pages (from-to)148-157
Number of pages10
JournalMagnetic Resonance in Medicine
Volume25
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

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Ferrosoferric Oxide
Dextrans
Monoclonal Antibodies
Lymphocytes
Streptavidin
Suspensions

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles. / Bulte, Jeff W; Hoekstra, Y.; Kamman, R. L.; Magin, R. L.; Webb, A. G.; Briggs, R. W.; Go, K. G.; Hulstaert, C. E.; Miltenyi, S.; The, T. H.; De Leij, L.

In: Magnetic Resonance in Medicine, Vol. 25, No. 1, 1992, p. 148-157.

Research output: Contribution to journalArticle

Bulte, JW, Hoekstra, Y, Kamman, RL, Magin, RL, Webb, AG, Briggs, RW, Go, KG, Hulstaert, CE, Miltenyi, S, The, TH & De Leij, L 1992, 'Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles', Magnetic Resonance in Medicine, vol. 25, no. 1, pp. 148-157. https://doi.org/10.1002/mrm.1910250115
Bulte, Jeff W ; Hoekstra, Y. ; Kamman, R. L. ; Magin, R. L. ; Webb, A. G. ; Briggs, R. W. ; Go, K. G. ; Hulstaert, C. E. ; Miltenyi, S. ; The, T. H. ; De Leij, L. / Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles. In: Magnetic Resonance in Medicine. 1992 ; Vol. 25, No. 1. pp. 148-157.
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