TY - JOUR
T1 - Specific immune induction following DNA-based immunization through in vivo transfection and activation of macrophages/antigen-presenting cells
AU - Chattergoon, Michael A.
AU - Robinson, Tara M.
AU - Boyer, Jean D.
AU - Weiner, David B.
PY - 1998/6/15
Y1 - 1998/6/15
N2 - The initiation of an adaptive immune response requires Ag presentation in combination with the appropriate activation signals. Classically, Ag presentation and immune activation occur in the lymph node and spleen, where a favorable organ architecture and rich cellular help can enhance the process. Recently, several investigators have reported the use of DNA expression cassettes to understood, plasmid inoculation represents a model system for studying immune function in response to invading pathogens. In this report, we demonstrate the presence of activated macrophages or dendritic cells in the blood lymphocyte pool and peripheral tissues of animals inoculation with DNA expression cassettes. These cells are directly transfected in vivo, present Ag, and play the surface proteins CD80 and CD86. Our studies indicate that these cells function as APC and can activate naive T lymphocytes. They may represent an important first step APC in genetic immunization and natural infection.
AB - The initiation of an adaptive immune response requires Ag presentation in combination with the appropriate activation signals. Classically, Ag presentation and immune activation occur in the lymph node and spleen, where a favorable organ architecture and rich cellular help can enhance the process. Recently, several investigators have reported the use of DNA expression cassettes to understood, plasmid inoculation represents a model system for studying immune function in response to invading pathogens. In this report, we demonstrate the presence of activated macrophages or dendritic cells in the blood lymphocyte pool and peripheral tissues of animals inoculation with DNA expression cassettes. These cells are directly transfected in vivo, present Ag, and play the surface proteins CD80 and CD86. Our studies indicate that these cells function as APC and can activate naive T lymphocytes. They may represent an important first step APC in genetic immunization and natural infection.
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M3 - Article
C2 - 9637479
AN - SCOPUS:0032526344
SN - 0022-1767
VL - 160
SP - 5707
EP - 5718
JO - Journal of Immunology
JF - Journal of Immunology
IS - 12
ER -