Specific immune induction following DNA-based immunization through in vivo transfection and activation of macrophages/antigen-presenting cells

Michael Anand Chattergoon, Tara M. Robinson, Jean D. Boyer, David B. Weiner

Research output: Contribution to journalArticle

Abstract

The initiation of an adaptive immune response requires Ag presentation in combination with the appropriate activation signals. Classically, Ag presentation and immune activation occur in the lymph node and spleen, where a favorable organ architecture and rich cellular help can enhance the process. Recently, several investigators have reported the use of DNA expression cassettes to understood, plasmid inoculation represents a model system for studying immune function in response to invading pathogens. In this report, we demonstrate the presence of activated macrophages or dendritic cells in the blood lymphocyte pool and peripheral tissues of animals inoculation with DNA expression cassettes. These cells are directly transfected in vivo, present Ag, and play the surface proteins CD80 and CD86. Our studies indicate that these cells function as APC and can activate naive T lymphocytes. They may represent an important first step APC in genetic immunization and natural infection.

Original languageEnglish (US)
Pages (from-to)5707-5718
Number of pages12
JournalJournal of Immunology
Volume160
Issue number12
StatePublished - Jun 15 1998
Externally publishedYes

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Macrophage Activation
Antigen-Presenting Cells
Transfection
Immunization
DNA
Adaptive Immunity
Dendritic Cells
Immune System
Membrane Proteins
Plasmids
Spleen
Lymph Nodes
Macrophages
Research Personnel
Lymphocytes
T-Lymphocytes
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Specific immune induction following DNA-based immunization through in vivo transfection and activation of macrophages/antigen-presenting cells. / Chattergoon, Michael Anand; Robinson, Tara M.; Boyer, Jean D.; Weiner, David B.

In: Journal of Immunology, Vol. 160, No. 12, 15.06.1998, p. 5707-5718.

Research output: Contribution to journalArticle

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