Specific globin mRNA accumulation was quantitated in several lines of K562 cells in the absence and the presence of hemin. Using specific cloned DNA probes, the amounts of ζ, α, ε and γ mRNAs were shown to be increased 2-3-fold in the presence of 20 μM hemin. No δ- or β-globin mRNAs were detectable in any of the lines. In one line, Bos, there was a marked decrease in ε-globin mRNA, which increased with hemin, although still to much lower levels than in the other lines. The decreased ε-globin mRNA accumulation in Bos is shown to be due to decreased ε-globin gene transcription.
|Original language||English (US)|
|Number of pages||6|
|State||Published - 1984|
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