Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA

Ilana Lotan, Philip Goelet, Ariela Gigi, Nathan Dascal

Research output: Contribution to journalArticlepeer-review

Abstract

Although the structure of rabbit skeletal muscle dihydropyridine (DHP) receptor, deduced from cDNA sequence, indicates that this protein is the channel-forming subunit of voltage-dependent calcium channel (VDCC), no functional proof for this prediction has been presented. Two DNA oligonucleotides complementary to DHP-receptor RNA sequences coding for putative membrane-spanning regions of the DHP receptor specifically suppress the expression of the DHP-sensitive VDCC from rabbit and rat heart in Xenopus oocytes. However, these oligonucleotides do not suppress the expression of the DHP-insensitive VDCC and of voltage-dependent sodium and potassium channels. Thus, the gene for DHP receptor of rabbit skeletal muscle is closely related, or identical to, a gene expressed in heart that encodes a component of the DHP-sensitive VDCC The DHP-sensitive and DHP-insensitive VDCCs are distinct molecular entities.

Original languageEnglish (US)
Pages (from-to)666-669
Number of pages4
JournalScience
Volume243
Issue number4891
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA'. Together they form a unique fingerprint.

Cite this