Specific αv integrin receptors modulate K1735 murine melanoma cell behavior

Expression of β3 integrins is increased in invasive melanoma. In this study we show that K1735 cell proliferation is enhanced by the expression of either β3 or a constitutively active Src. We investigated possible modulators of FN matrix assembly and found that matrix metalloproteinase 2 (MMP2) was activated by αvβ3. αvβ3 integrin was localized to focal contacts whereas αvβ5 was peripherally distributed. MMP2 was also activated by expression of CASrc. MMP2 activation inversely correlated with FN matrix assembly, in that it dramatically reduced the organization of a FN matrix. K1735 cell migration on VN and invasion through a reconstituted basement membrane were decreased in the presence of anti-MMP2 antibodies. These results demonstrate that the expression of the αvβ3 complex modulates melanoma cell behavior including activation of Src, organization of the cytoskeleton, assembly of the extracellular matrix, cell motility, and activation of MMP2.