Species-specific and drug-specific differences in susceptibility of candida biofilms to echinocandins: Characterization of less common bloodstream isolates

Maria Simitsopoulou, Pavla Peshkova, Efthymia Tasina, Aspasia Katragkou, Daniela Kyrpitzi, Aristea Velegraki, Thomas J. Walsh, Emmanuel Roilides

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Candida species other than Candida albicans are increasingly recognized as causes of biofilm-associated infections. This is a comprehensive study that compared the in vitro activities of all three echinocandins against biofilms formed by different common and infrequently identified Candida isolates. We determined the activities of anidulafungin (ANID), caspofungin (CAS), and micafungin (MFG) against planktonic cells and biofilms of bloodstream isolates of C. albicans (15 strains), Candida parapsilosis (6 strains), Candida lusitaniae (16 strains), Candida guilliermondii (5 strains), and Candida krusei (12 strains) by XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5- carboxanilide] assay. Planktonic and biofilm MICs were defined as>50% fungal damage. Planktonic cells of all Candida species were susceptible to the three echinocandins, with MICs of≤1 mg/liter. By comparison, differences in the MIC profiles of biofilms in response to echinocandins existed among the Candida species. Thus, C. lusitaniae and C. guilliermondii biofilms were highly recalcitrant to all echinocandins, with MICs of ≥32 mg/ liter. In contrast, the MICs of all three echinocandins for C. albicans and C. krusei biofilms were relatively low (MICs ≤ 1 mg/ liter). While echinocandins exhibited generally high MICs against C. parapsilosis biofilms, MFG exhibited the lowest MICs against these isolates (4 mg/liter). A paradoxical growth effect was observed with CAS concentrations ranging from 8 to 64 mg/ liter against C. albicans and C. parapsilosis biofilms but not against C. krusei, C. lusitaniae, or C. guilliermondii. While nonalbicans Candida planktonic cells were susceptible to all echinocandins, there were drug- and species-specific differences in susceptibility among biofilms of the various Candida species, with C. lusitaniae and C. guilliermondii exhibiting profiles of high MICs of the three echinocandins.

Original languageEnglish (US)
Pages (from-to)2562-2570
Number of pages9
JournalAntimicrobial agents and chemotherapy
Issue number6
StatePublished - Jun 2013
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases


Dive into the research topics of 'Species-specific and drug-specific differences in susceptibility of candida biofilms to echinocandins: Characterization of less common bloodstream isolates'. Together they form a unique fingerprint.

Cite this