Species of Metarhizium anisopliae complex implicated in human infections

Retrospective sequencing study

C. Nourrisson, D. Dupont, R. A. Lavergne, J. Dorin, F. Forouzanfar, J. Denis, Kristina Weeks, R. Joubert, F. Chiambaretta, T. Bourcier, S. Roux, A. Sénéchal, G. Benaïm, M. Wallon, E. Candolfi, V. Letscher-Bru, P. Poirier, M. Sabou

Research output: Contribution to journalArticle

Abstract

Objectives: Fungi belonging to the Metarhizium anisopliae complex comprise ubiquitous arthropod pathogenic moulds used as mycopesticides. Rare cases of human infections due to M. anisopliae have been reported. We hypothesize misidentifications of fungal strains implicated in these cases or used in mycopesticides. Methods: A review of the literature was conducted to identify previously published cases. We collected some of these previous described strains and reported new cases, and a French mycopesticide containing M. anisopliae. All identifications were performed based on elongation factor-1α gene sequencing. Results: We report eight new cases of Metarhizium infection in humans (three from France and five from Australia). The strains isolated from these cases, and three others from already published cases and reported as M. anisopliae, were molecularly identified based on elongation factor-1α ( Ef1-α) gene sequencing as follows: Metarhizium robertsii (six), Metarhizium guizhouense (three), Metarhizium brunneum (one) and Metarhizium pingshaense (one). Conclusions: In this study, we report new human cases of Metarhizium infections, and, based on Ef-1α gene sequencing, we demonstrate the misidentification of species in case reports. We also correct the species identification of a strain reported as M. anisopliae used in a commercially available mycopesticide. According to our results, none of the strains from the human infection reports reviewed belongs to the species M. anisopliae.

Original languageEnglish (US)
JournalClinical Microbiology and Infection
DOIs
StateAccepted/In press - Feb 19 2017
Externally publishedYes

Fingerprint

Metarhizium
Retrospective Studies
Infection
Peptide Elongation Factor 1
Fungi
Genes
Arthropods
France

Keywords

  • Elongation factor 1-α
  • Internal transcribed spacer
  • Metarhizium
  • Metarhizium anisopliae
  • Metarhizium robertsii
  • Ocular fungal infection
  • Sequencing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Nourrisson, C., Dupont, D., Lavergne, R. A., Dorin, J., Forouzanfar, F., Denis, J., ... Sabou, M. (Accepted/In press). Species of Metarhizium anisopliae complex implicated in human infections: Retrospective sequencing study. Clinical Microbiology and Infection. https://doi.org/10.1016/j.cmi.2017.05.001

Species of Metarhizium anisopliae complex implicated in human infections : Retrospective sequencing study. / Nourrisson, C.; Dupont, D.; Lavergne, R. A.; Dorin, J.; Forouzanfar, F.; Denis, J.; Weeks, Kristina; Joubert, R.; Chiambaretta, F.; Bourcier, T.; Roux, S.; Sénéchal, A.; Benaïm, G.; Wallon, M.; Candolfi, E.; Letscher-Bru, V.; Poirier, P.; Sabou, M.

In: Clinical Microbiology and Infection, 19.02.2017.

Research output: Contribution to journalArticle

Nourrisson, C, Dupont, D, Lavergne, RA, Dorin, J, Forouzanfar, F, Denis, J, Weeks, K, Joubert, R, Chiambaretta, F, Bourcier, T, Roux, S, Sénéchal, A, Benaïm, G, Wallon, M, Candolfi, E, Letscher-Bru, V, Poirier, P & Sabou, M 2017, 'Species of Metarhizium anisopliae complex implicated in human infections: Retrospective sequencing study', Clinical Microbiology and Infection. https://doi.org/10.1016/j.cmi.2017.05.001
Nourrisson, C. ; Dupont, D. ; Lavergne, R. A. ; Dorin, J. ; Forouzanfar, F. ; Denis, J. ; Weeks, Kristina ; Joubert, R. ; Chiambaretta, F. ; Bourcier, T. ; Roux, S. ; Sénéchal, A. ; Benaïm, G. ; Wallon, M. ; Candolfi, E. ; Letscher-Bru, V. ; Poirier, P. ; Sabou, M. / Species of Metarhizium anisopliae complex implicated in human infections : Retrospective sequencing study. In: Clinical Microbiology and Infection. 2017.
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abstract = "Objectives: Fungi belonging to the Metarhizium anisopliae complex comprise ubiquitous arthropod pathogenic moulds used as mycopesticides. Rare cases of human infections due to M. anisopliae have been reported. We hypothesize misidentifications of fungal strains implicated in these cases or used in mycopesticides. Methods: A review of the literature was conducted to identify previously published cases. We collected some of these previous described strains and reported new cases, and a French mycopesticide containing M. anisopliae. All identifications were performed based on elongation factor-1α gene sequencing. Results: We report eight new cases of Metarhizium infection in humans (three from France and five from Australia). The strains isolated from these cases, and three others from already published cases and reported as M. anisopliae, were molecularly identified based on elongation factor-1α ( Ef1-α) gene sequencing as follows: Metarhizium robertsii (six), Metarhizium guizhouense (three), Metarhizium brunneum (one) and Metarhizium pingshaense (one). Conclusions: In this study, we report new human cases of Metarhizium infections, and, based on Ef-1α gene sequencing, we demonstrate the misidentification of species in case reports. We also correct the species identification of a strain reported as M. anisopliae used in a commercially available mycopesticide. According to our results, none of the strains from the human infection reports reviewed belongs to the species M. anisopliae.",
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T2 - Retrospective sequencing study

AU - Nourrisson, C.

AU - Dupont, D.

AU - Lavergne, R. A.

AU - Dorin, J.

AU - Forouzanfar, F.

AU - Denis, J.

AU - Weeks, Kristina

AU - Joubert, R.

AU - Chiambaretta, F.

AU - Bourcier, T.

AU - Roux, S.

AU - Sénéchal, A.

AU - Benaïm, G.

AU - Wallon, M.

AU - Candolfi, E.

AU - Letscher-Bru, V.

AU - Poirier, P.

AU - Sabou, M.

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N2 - Objectives: Fungi belonging to the Metarhizium anisopliae complex comprise ubiquitous arthropod pathogenic moulds used as mycopesticides. Rare cases of human infections due to M. anisopliae have been reported. We hypothesize misidentifications of fungal strains implicated in these cases or used in mycopesticides. Methods: A review of the literature was conducted to identify previously published cases. We collected some of these previous described strains and reported new cases, and a French mycopesticide containing M. anisopliae. All identifications were performed based on elongation factor-1α gene sequencing. Results: We report eight new cases of Metarhizium infection in humans (three from France and five from Australia). The strains isolated from these cases, and three others from already published cases and reported as M. anisopliae, were molecularly identified based on elongation factor-1α ( Ef1-α) gene sequencing as follows: Metarhizium robertsii (six), Metarhizium guizhouense (three), Metarhizium brunneum (one) and Metarhizium pingshaense (one). Conclusions: In this study, we report new human cases of Metarhizium infections, and, based on Ef-1α gene sequencing, we demonstrate the misidentification of species in case reports. We also correct the species identification of a strain reported as M. anisopliae used in a commercially available mycopesticide. According to our results, none of the strains from the human infection reports reviewed belongs to the species M. anisopliae.

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KW - Sequencing

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