TY - JOUR
T1 - Species of Metarhizium anisopliae complex implicated in human infections
T2 - retrospective sequencing study
AU - Nourrisson, C.
AU - Dupont, D.
AU - Lavergne, R. A.
AU - Dorin, J.
AU - Forouzanfar, F.
AU - Denis, J.
AU - Weeks, K.
AU - Joubert, R.
AU - Chiambaretta, F.
AU - Bourcier, T.
AU - Roux, S.
AU - Sénéchal, A.
AU - Benaïm, G.
AU - Wallon, M.
AU - Candolfi, E.
AU - Letscher-Bru, V.
AU - Poirier, P.
AU - Sabou, M.
N1 - Publisher Copyright:
© 2017
PY - 2017/12
Y1 - 2017/12
N2 - Objectives Fungi belonging to the Metarhizium anisopliae complex comprise ubiquitous arthropod pathogenic moulds used as mycopesticides. Rare cases of human infections due to M. anisopliae have been reported. We hypothesize misidentifications of fungal strains implicated in these cases or used in mycopesticides. Methods A review of the literature was conducted to identify previously published cases. We collected some of these previous described strains and reported new cases, and a French mycopesticide containing M. anisopliae. All identifications were performed based on elongation factor-1α gene sequencing. Results We report eight new cases of Metarhizium infection in humans (three from France and five from Australia). The strains isolated from these cases, and three others from already published cases and reported as M. anisopliae, were molecularly identified based on elongation factor-1α (Ef1-α) gene sequencing as follows: Metarhizium robertsii (six), Metarhizium guizhouense (three), Metarhizium brunneum (one) and Metarhizium pingshaense (one). Conclusions In this study, we report new human cases of Metarhizium infections, and, based on Ef-1α gene sequencing, we demonstrate the misidentification of species in case reports. We also correct the species identification of a strain reported as M. anisopliae used in a commercially available mycopesticide. According to our results, none of the strains from the human infection reports reviewed belongs to the species M. anisopliae.
AB - Objectives Fungi belonging to the Metarhizium anisopliae complex comprise ubiquitous arthropod pathogenic moulds used as mycopesticides. Rare cases of human infections due to M. anisopliae have been reported. We hypothesize misidentifications of fungal strains implicated in these cases or used in mycopesticides. Methods A review of the literature was conducted to identify previously published cases. We collected some of these previous described strains and reported new cases, and a French mycopesticide containing M. anisopliae. All identifications were performed based on elongation factor-1α gene sequencing. Results We report eight new cases of Metarhizium infection in humans (three from France and five from Australia). The strains isolated from these cases, and three others from already published cases and reported as M. anisopliae, were molecularly identified based on elongation factor-1α (Ef1-α) gene sequencing as follows: Metarhizium robertsii (six), Metarhizium guizhouense (three), Metarhizium brunneum (one) and Metarhizium pingshaense (one). Conclusions In this study, we report new human cases of Metarhizium infections, and, based on Ef-1α gene sequencing, we demonstrate the misidentification of species in case reports. We also correct the species identification of a strain reported as M. anisopliae used in a commercially available mycopesticide. According to our results, none of the strains from the human infection reports reviewed belongs to the species M. anisopliae.
KW - Elongation factor 1-α
KW - Internal transcribed spacer
KW - Metarhizium
KW - Metarhizium anisopliae
KW - Metarhizium robertsii
KW - Ocular fungal infection
KW - Sequencing
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U2 - 10.1016/j.cmi.2017.05.001
DO - 10.1016/j.cmi.2017.05.001
M3 - Article
C2 - 28487164
AN - SCOPUS:85021207200
SN - 1198-743X
VL - 23
SP - 994
EP - 999
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
IS - 12
ER -