Specialized membrane domains for water transport in glial cells

High- resolution immunogold cytochemistry of aquaporin-4 in rat brain

So Nielsen, Erlend Arnulf Nagelhus, Mahmood Amiry-Moghaddam, Charles Bourque, Peter C Agre, Ole Retter Ottersen

Research output: Contribution to journalArticle

Abstract

Membrane water transport is critically involved in brain volume homeostasis and in the pathogenesis of brain edema. The cDNA encoding aquaporin-4 (AQP4) water channel protein was recently isolated from rat brain. We used immunocytochemistry and high-resolution immunogold electron microscopy to identify the cells and membrane domains that mediate water flux through AQP4. The AQP4 protein is abundant in glial cells bordering the subarachnoidal space, ventricles, and brood vessels. AQP4 is also abundant in osmosensory areas, including the supraoptic nucleus and subfornical organ. Immunogold analysis demonstrated that AQP4 is restricted to glial membranes and to subpopulations of ependymal cells. AQP4 is particularly strongly expressed in glial membranes that are in direct contact with capillaries and pia. The highly polarized AQP4 expression indicates that these cells are equipped with specific membrane domains that are specialized for water transport, thereby mediating the flow of water between glial cells and the cavities filled with CSF and the intravascular space.

Original languageEnglish (US)
Pages (from-to)171-180
Number of pages10
JournalJournal of Neuroscience
Volume17
Issue number1
StatePublished - 1997

Fingerprint

Aquaporin 4
Histocytochemistry
Neuroglia
Membranes
Water
Brain
Subfornical Organ
Supraoptic Nucleus
Aquaporins
Brain Edema
Electron Microscopy
Homeostasis
Complementary DNA
Immunohistochemistry
Cell Membrane

Keywords

  • aquaporin-4 water channel
  • brain water permeability
  • CSF
  • ependymal cells
  • glial cells
  • immunogold electron microscopy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Specialized membrane domains for water transport in glial cells : High- resolution immunogold cytochemistry of aquaporin-4 in rat brain. / Nielsen, So; Nagelhus, Erlend Arnulf; Amiry-Moghaddam, Mahmood; Bourque, Charles; Agre, Peter C; Ottersen, Ole Retter.

In: Journal of Neuroscience, Vol. 17, No. 1, 1997, p. 171-180.

Research output: Contribution to journalArticle

Nielsen, S, Nagelhus, EA, Amiry-Moghaddam, M, Bourque, C, Agre, PC & Ottersen, OR 1997, 'Specialized membrane domains for water transport in glial cells: High- resolution immunogold cytochemistry of aquaporin-4 in rat brain', Journal of Neuroscience, vol. 17, no. 1, pp. 171-180.
Nielsen, So ; Nagelhus, Erlend Arnulf ; Amiry-Moghaddam, Mahmood ; Bourque, Charles ; Agre, Peter C ; Ottersen, Ole Retter. / Specialized membrane domains for water transport in glial cells : High- resolution immunogold cytochemistry of aquaporin-4 in rat brain. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 1. pp. 171-180.
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