Spatiotemporal distribution of fibrinogen in marmoset and human inflammatory demyelination

Nathanael J. Lee, Seung Kwon Ha, Pascal Sati, Martina Absinta, Nicholas J. Luciano, Jennifer A. Lefeuvre, Matthew K. Schindler, Emily C. Leibovitch, Jae Kyu Ryu, Mark A. Petersen, Afonso C. Silva, Steven Jacobson, Katerina Akassoglou, Daniel S. Reich

Research output: Contribution to journalArticle

Abstract

Multiple sclerosis is an inflammatory demyelinating disease of the central nervous system. Although it has been extensively studied, the proximate trigger of the immune response remains uncertain. Experimental autoimmune encephalomyelitis in the common marmoset recapitulates many radiological and pathological features of focal multiple sclerosis lesions in the cerebral white matter, unlike traditional experimental autoimmune encephalomyelitis in rodents. This provides an opportunity to investigate how lesions form as well as the relative timing of factors involved in lesion pathogenesis, especially during early stages of the disease. We used MRI to track experimental autoimmune encephalomyelitis lesions in vivo to determine their age, stage of development, and location, and we assessed the corresponding histopathology post-mortem. We focused on the plasma protein fibrinogen - A marker for blood-brain barrier leakage that has also been linked to a pathogenic role in inflammatory demyelinating lesion development. We show that fibrinogen has a specific spatiotemporal deposition pattern, apparently deriving from the central vein in early experimental autoimmune encephalomyelitis lesions <6 weeks old, and preceding both demyelination and visible gadolinium enhancement on MRI. Thus, fibrinogen leakage is one of the earliest detectable events in lesion pathogenesis. In slightly older lesions, fibrinogen is found inside microglia/macrophages, suggesting rapid phagocytosis. Quantification demonstrates positive correlation of fibrinogen deposition with accumulation of inflammatory cells, including microglia/macrophages and T cells. The peak of fibrinogen deposition coincides with the onset of demyelination and axonal loss. In samples from chronic multiple sclerosis cases, fibrinogen was found at the edge of chronic active lesions, which have ongoing demyelination and inflammation, but not in inactive lesions, suggesting that fibrinogen may play a role in sustained inflammation even in the chronic setting. In summary, our data support the notion that fibrinogen is a key player in the early pathogenesis, as well as sustained inflammation, of inflammatory demyelinating lesions.

Original languageEnglish (US)
Pages (from-to)1637-1649
Number of pages13
JournalBrain
Volume141
Issue number6
DOIs
StatePublished - Jun 1 2018
Externally publishedYes

Fingerprint

Callithrix
Demyelinating Diseases
Fibrinogen
Autoimmune Experimental Encephalomyelitis
Multiple Sclerosis
Microglia
Inflammation
Macrophages
Gadolinium
Blood-Brain Barrier
Phagocytosis
Blood Proteins
Veins
Rodentia
Central Nervous System
T-Lymphocytes

Keywords

  • blood-brain barrier
  • common marmosets (Callithrix jacchus)
  • experimental autoimmune encephalomyelitis
  • fibrinogen
  • multiple sclerosis

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Lee, N. J., Ha, S. K., Sati, P., Absinta, M., Luciano, N. J., Lefeuvre, J. A., ... Reich, D. S. (2018). Spatiotemporal distribution of fibrinogen in marmoset and human inflammatory demyelination. Brain, 141(6), 1637-1649. https://doi.org/10.1093/brain/awy082

Spatiotemporal distribution of fibrinogen in marmoset and human inflammatory demyelination. / Lee, Nathanael J.; Ha, Seung Kwon; Sati, Pascal; Absinta, Martina; Luciano, Nicholas J.; Lefeuvre, Jennifer A.; Schindler, Matthew K.; Leibovitch, Emily C.; Ryu, Jae Kyu; Petersen, Mark A.; Silva, Afonso C.; Jacobson, Steven; Akassoglou, Katerina; Reich, Daniel S.

In: Brain, Vol. 141, No. 6, 01.06.2018, p. 1637-1649.

Research output: Contribution to journalArticle

Lee, NJ, Ha, SK, Sati, P, Absinta, M, Luciano, NJ, Lefeuvre, JA, Schindler, MK, Leibovitch, EC, Ryu, JK, Petersen, MA, Silva, AC, Jacobson, S, Akassoglou, K & Reich, DS 2018, 'Spatiotemporal distribution of fibrinogen in marmoset and human inflammatory demyelination', Brain, vol. 141, no. 6, pp. 1637-1649. https://doi.org/10.1093/brain/awy082
Lee, Nathanael J. ; Ha, Seung Kwon ; Sati, Pascal ; Absinta, Martina ; Luciano, Nicholas J. ; Lefeuvre, Jennifer A. ; Schindler, Matthew K. ; Leibovitch, Emily C. ; Ryu, Jae Kyu ; Petersen, Mark A. ; Silva, Afonso C. ; Jacobson, Steven ; Akassoglou, Katerina ; Reich, Daniel S. / Spatiotemporal distribution of fibrinogen in marmoset and human inflammatory demyelination. In: Brain. 2018 ; Vol. 141, No. 6. pp. 1637-1649.
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abstract = "Multiple sclerosis is an inflammatory demyelinating disease of the central nervous system. Although it has been extensively studied, the proximate trigger of the immune response remains uncertain. Experimental autoimmune encephalomyelitis in the common marmoset recapitulates many radiological and pathological features of focal multiple sclerosis lesions in the cerebral white matter, unlike traditional experimental autoimmune encephalomyelitis in rodents. This provides an opportunity to investigate how lesions form as well as the relative timing of factors involved in lesion pathogenesis, especially during early stages of the disease. We used MRI to track experimental autoimmune encephalomyelitis lesions in vivo to determine their age, stage of development, and location, and we assessed the corresponding histopathology post-mortem. We focused on the plasma protein fibrinogen - A marker for blood-brain barrier leakage that has also been linked to a pathogenic role in inflammatory demyelinating lesion development. We show that fibrinogen has a specific spatiotemporal deposition pattern, apparently deriving from the central vein in early experimental autoimmune encephalomyelitis lesions <6 weeks old, and preceding both demyelination and visible gadolinium enhancement on MRI. Thus, fibrinogen leakage is one of the earliest detectable events in lesion pathogenesis. In slightly older lesions, fibrinogen is found inside microglia/macrophages, suggesting rapid phagocytosis. Quantification demonstrates positive correlation of fibrinogen deposition with accumulation of inflammatory cells, including microglia/macrophages and T cells. The peak of fibrinogen deposition coincides with the onset of demyelination and axonal loss. In samples from chronic multiple sclerosis cases, fibrinogen was found at the edge of chronic active lesions, which have ongoing demyelination and inflammation, but not in inactive lesions, suggesting that fibrinogen may play a role in sustained inflammation even in the chronic setting. In summary, our data support the notion that fibrinogen is a key player in the early pathogenesis, as well as sustained inflammation, of inflammatory demyelinating lesions.",
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