Spatially-multiplexed protein separations enabled by multifunctional photopolymerized gels

Shuang Yang, Jikun Liu, Don L. DeVoe

Research output: Contribution to conferencePaper

Abstract

A 2-D microfluidic chip combining isoelectric focusing (IEF) and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) using photopolymerized polyacrylamide (PAAm) is described. In addition to serving as a highlyresolving separation medium for gel electrophoresis, the PAAm gels are used to enable the efficient isolation of different on-chip media including anolyte, catholyte, and sample/ampholyte solutions for IEF. The gel plugs are demonstrated as on-chip reagent containers, holding defined quantities of SDS for in situ SDS-protein complexation, and enabling the use of a discontinuous buffer system for sample band sharpening during SDS-PAGE.The new chip design also provides lossless sample injection and the optimized backbiasing to minimize sample tailing and leakage.

Original languageEnglish (US)
Pages173-175
Number of pages3
StatePublished - Jan 1 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • 2-D PAGE
  • Backbiasing
  • IEF
  • PMMA
  • Polyacrylamide

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Yang, S., Liu, J., & DeVoe, D. L. (2008). Spatially-multiplexed protein separations enabled by multifunctional photopolymerized gels. 173-175. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.