Abstract
A 2-D microfluidic chip combining isoelectric focusing (IEF) and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) using photopolymerized polyacrylamide (PAAm) is described. In addition to serving as a highlyresolving separation medium for gel electrophoresis, the PAAm gels are used to enable the efficient isolation of different on-chip media including anolyte, catholyte, and sample/ampholyte solutions for IEF. The gel plugs are demonstrated as on-chip reagent containers, holding defined quantities of SDS for in situ SDS-protein complexation, and enabling the use of a discontinuous buffer system for sample band sharpening during SDS-PAGE.The new chip design also provides lossless sample injection and the optimized backbiasing to minimize sample tailing and leakage.
| Original language | English (US) |
|---|---|
| Title of host publication | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 173-175 |
| Number of pages | 3 |
| Publication status | Published - 2008 |
| Externally published | Yes |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008 |
Other
| Other | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
|---|---|
| Country | United States |
| City | San Diego, CA |
| Period | 10/12/08 → 10/16/08 |
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Keywords
- 2-D PAGE
- Backbiasing
- IEF
- PMMA
- Polyacrylamide
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering