Spatially-multiplexed protein separations enabled by multifunctional photopolymerized gels

Shuang Yang, Jikun Liu, Don L. DeVoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A 2-D microfluidic chip combining isoelectric focusing (IEF) and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) using photopolymerized polyacrylamide (PAAm) is described. In addition to serving as a highlyresolving separation medium for gel electrophoresis, the PAAm gels are used to enable the efficient isolation of different on-chip media including anolyte, catholyte, and sample/ampholyte solutions for IEF. The gel plugs are demonstrated as on-chip reagent containers, holding defined quantities of SDS for in situ SDS-protein complexation, and enabling the use of a discontinuous buffer system for sample band sharpening during SDS-PAGE.The new chip design also provides lossless sample injection and the optimized backbiasing to minimize sample tailing and leakage.

Original languageEnglish (US)
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages173-175
Number of pages3
Publication statusPublished - 2008
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

    Fingerprint

Keywords

  • 2-D PAGE
  • Backbiasing
  • IEF
  • PMMA
  • Polyacrylamide

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Yang, S., Liu, J., & DeVoe, D. L. (2008). Spatially-multiplexed protein separations enabled by multifunctional photopolymerized gels. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 173-175). Chemical and Biological Microsystems Society.