TY - JOUR
T1 - Spatial organization of transcription in bacterial cells
AU - Weng, Xiaoli
AU - Xiao, Jie
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/7
Y1 - 2014/7
N2 - Prokaryotic transcription has been extensively studied over the past half a century. However, there often exists a gap between the structural, mechanistic description of transcription obtained from in vitro biochemical studies, and the cellular, phenomenological observations from in vivo genetic studies. It is now accepted that a living bacterial cell is a complex entity; the heterogeneous cellular environment is drastically different from the homogenous, well-mixed situation in vitro. Where molecules are inside a cell may be important for their function; hence, the spatial organization of different molecular components may provide a new means of transcription regulation in vivo, possibly bridging this gap. In this review, we survey current evidence for the spatial organization of four major components of transcription [genes, transcription factors, RNA polymerase (RNAP) and RNAs] and critically analyze their biological significance.
AB - Prokaryotic transcription has been extensively studied over the past half a century. However, there often exists a gap between the structural, mechanistic description of transcription obtained from in vitro biochemical studies, and the cellular, phenomenological observations from in vivo genetic studies. It is now accepted that a living bacterial cell is a complex entity; the heterogeneous cellular environment is drastically different from the homogenous, well-mixed situation in vitro. Where molecules are inside a cell may be important for their function; hence, the spatial organization of different molecular components may provide a new means of transcription regulation in vivo, possibly bridging this gap. In this review, we survey current evidence for the spatial organization of four major components of transcription [genes, transcription factors, RNA polymerase (RNAP) and RNAs] and critically analyze their biological significance.
KW - Chromosome conformation capture
KW - Prokaryotic transcription
KW - RNA polymerase
KW - Single molecule
KW - Spatial organization
KW - Transcription factors
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U2 - 10.1016/j.tig.2014.04.008
DO - 10.1016/j.tig.2014.04.008
M3 - Review article
C2 - 24862529
AN - SCOPUS:84903436527
VL - 30
SP - 287
EP - 297
JO - Trends in Genetics
JF - Trends in Genetics
SN - 0168-9525
IS - 7
ER -