Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway

Srivathsan Adivarahan, Nathan Livingston, Beth Nicholson, Samir Rahman, Bin Wu, Olivia S. Rissland, Daniel Zenklusen

Research output: Contribution to journalArticle

Abstract

mRNAs form ribonucleoprotein complexes (mRNPs) by association with proteins that are crucial for mRNA metabolism. While the mRNP proteome has been well characterized, little is known about mRNP organization. Using a single-molecule approach, we show that mRNA conformation changes depending on its cellular localization and translational state. Compared to nuclear mRNPs and lncRNPs, association with ribosomes decompacts individual mRNAs, while pharmacologically dissociating ribosomes or sequestering them into stress granules leads to increased compaction. Moreover, translating mRNAs rarely show co-localized 5' and 3' ends, indicating either that mRNAs are not translated in a closed-loop configuration, or that mRNA circularization is transient, suggesting that a stable closed-loop conformation is not a universal state for all translating mRNAs.

Original languageEnglish (US)
Pages (from-to)727-738
Number of pages12
JournalMolecular Cell
Volume72
Issue number4
DOIs
StatePublished - Nov 15 2018

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Ribonucleoproteins
Gene Expression
Messenger RNA
Ribosomes
Proteome

Keywords

  • closed-loop translation
  • long non-coding RNAs
  • mRNP organization
  • RNA compaction
  • RNA imaging
  • RNA structure
  • smFISH
  • stress granules
  • super resolution microscopy
  • translation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Adivarahan, S., Livingston, N., Nicholson, B., Rahman, S., Wu, B., Rissland, O. S., & Zenklusen, D. (2018). Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway. Molecular Cell, 72(4), 727-738. https://doi.org/10.1016/j.molcel.2018.10.010

Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway. / Adivarahan, Srivathsan; Livingston, Nathan; Nicholson, Beth; Rahman, Samir; Wu, Bin; Rissland, Olivia S.; Zenklusen, Daniel.

In: Molecular Cell, Vol. 72, No. 4, 15.11.2018, p. 727-738.

Research output: Contribution to journalArticle

Adivarahan, S, Livingston, N, Nicholson, B, Rahman, S, Wu, B, Rissland, OS & Zenklusen, D 2018, 'Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway', Molecular Cell, vol. 72, no. 4, pp. 727-738. https://doi.org/10.1016/j.molcel.2018.10.010
Adivarahan S, Livingston N, Nicholson B, Rahman S, Wu B, Rissland OS et al. Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway. Molecular Cell. 2018 Nov 15;72(4):727-738. https://doi.org/10.1016/j.molcel.2018.10.010
Adivarahan, Srivathsan ; Livingston, Nathan ; Nicholson, Beth ; Rahman, Samir ; Wu, Bin ; Rissland, Olivia S. ; Zenklusen, Daniel. / Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway. In: Molecular Cell. 2018 ; Vol. 72, No. 4. pp. 727-738.
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