Spatial mapping of nitric oxide generation in the ischemic heart using electron paramagnetic resonance imaging

Periannan Kuppusamy, Penghai Wang, Alexandre Samouilov, Jay L. Zweier

Research output: Contribution to journalArticle

Abstract

Recently, it has been shown that rat hearts subjected to global ischemia generate nitric oxide (NO) and that a significant portion of it is generated by the reduction of nitrite under the acidic and reducing conditions that occur during myocardial ischemia [Zweier, Wang, Samouilov, Kuppusamy, Nature Med. 1, 804-809 (1995)]. In the present study it is further attempted to map the spatial distributions of the NO generation in the ischemic myocardium using L-band electron paramagnetic resonance imaging. Rat hearts were loaded with 10 mM nitrite end subjected to global no-flow ischemia, during which time a series of three-dimensional spatial electron paramagnetic resonance (EPR) images of the distribution of NO were obtained using the NO trap bis(N-methyl-D-glucamine dithiocarbamate)iron(II). The images clearly showed that NO is formed throughout the myocardium. Kinetic experiments showed that maximum NO generation and trapping occur at the midmyocardium and spread out to endocardium and epicardium of the left ventricle. The magnitude of generation in the RV myocardium is four- to fivefold lower than in the LV.

Original languageEnglish (US)
Pages (from-to)212-218
Number of pages7
JournalMagnetic Resonance in Medicine
Volume36
Issue number2
StatePublished - Aug 1996

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Electron Spin Resonance Spectroscopy
Nitric Oxide
Myocardium
Nitrites
Ischemia
Endocardium
Pericardium
Heart Ventricles
Myocardial Ischemia

Keywords

  • acidosis
  • EPR imaging
  • ischemia
  • nitric oxide
  • nitrite
  • rat heart

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Spatial mapping of nitric oxide generation in the ischemic heart using electron paramagnetic resonance imaging. / Kuppusamy, Periannan; Wang, Penghai; Samouilov, Alexandre; Zweier, Jay L.

In: Magnetic Resonance in Medicine, Vol. 36, No. 2, 08.1996, p. 212-218.

Research output: Contribution to journalArticle

Kuppusamy, Periannan ; Wang, Penghai ; Samouilov, Alexandre ; Zweier, Jay L. / Spatial mapping of nitric oxide generation in the ischemic heart using electron paramagnetic resonance imaging. In: Magnetic Resonance in Medicine. 1996 ; Vol. 36, No. 2. pp. 212-218.
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