Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

Erin E H Tran; James A. Simmons; Alberto Bartesaghi; Charles J. Shoemaker; Elizabeth Nelson; Judith M. White; Sriram Subramaniam

doi:10.1128/JVI.00870-14

Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

Erin E H Tran, James A. Simmons, Alberto Bartesaghi, Charles J. Shoemaker, Elizabeth Nelson, Judith M. White, Sriram Subramaniam

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

Original language	English (US)
Pages (from-to)	10958-10962
Number of pages	5
Journal	Journal of Virology
Volume	88
Issue number	18
DOIs	https://doi.org/10.1128/JVI.00870-14
State	Published - 2014
Externally published	Yes

ASJC Scopus subject areas

Immunology
Virology
General Medicine

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10.1128/JVI.00870-14

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abstract = "The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.",

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AU - Simmons, James A.

AU - Bartesaghi, Alberto

AU - Shoemaker, Charles J.

AU - Nelson, Elizabeth

AU - White, Judith M.

AU - Subramaniam, Sriram

PY - 2014

Y1 - 2014

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AB - The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

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Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

Abstract

ASJC Scopus subject areas

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