Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

Erin E H Tran, James A. Simmons, Alberto Bartesaghi, Charles J. Shoemaker, Elizabeth Nelson, Judith M. White, Sriram Subramaniam

Research output: Contribution to journalArticle

Abstract

The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

Original languageEnglish (US)
Pages (from-to)10958-10962
Number of pages5
JournalJournal of Virology
Volume88
Issue number18
DOIs
StatePublished - 2014
Externally publishedYes

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Ebolavirus
Electron Microscope Tomography
mucins
tomography
Mucins
glycoproteins
Glycoproteins
electrons
immune evasion
Immune Evasion
Virus Internalization
virus-like particles
Virion
cells

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Medicine(all)

Cite this

Tran, E. E. H., Simmons, J. A., Bartesaghi, A., Shoemaker, C. J., Nelson, E., White, J. M., & Subramaniam, S. (2014). Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography. Journal of Virology, 88(18), 10958-10962. https://doi.org/10.1128/JVI.00870-14

Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography. / Tran, Erin E H; Simmons, James A.; Bartesaghi, Alberto; Shoemaker, Charles J.; Nelson, Elizabeth; White, Judith M.; Subramaniam, Sriram.

In: Journal of Virology, Vol. 88, No. 18, 2014, p. 10958-10962.

Research output: Contribution to journalArticle

Tran, EEH, Simmons, JA, Bartesaghi, A, Shoemaker, CJ, Nelson, E, White, JM & Subramaniam, S 2014, 'Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography', Journal of Virology, vol. 88, no. 18, pp. 10958-10962. https://doi.org/10.1128/JVI.00870-14
Tran, Erin E H ; Simmons, James A. ; Bartesaghi, Alberto ; Shoemaker, Charles J. ; Nelson, Elizabeth ; White, Judith M. ; Subramaniam, Sriram. / Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography. In: Journal of Virology. 2014 ; Vol. 88, No. 18. pp. 10958-10962.
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