Spatial localization in NMR spectroscopy in vivo

Research output: Contribution to journalArticle

Abstract

Spatial localization techniques are necessary for in vivo NMR spectroscopy involving heterogeneous organisms. Localization by surface coil NMR detection alone is generally inadequate for deep-lying organs due to contaminating signals from intervening surface tissues. However, localization to preselected planar volumes can be accomplished using a single selective excitation pulse in the presence of a pulsed magnetic field gradient, yielding depth-resolved surface coil spectra (DRESS). Within selected planes, DRESS are spatially restricted by the surface coil sensitivity profiles to disk-shaped volumes whose radii increase with depth, notwithstanding variations in the NMR signal density distribution. Nevertheless, DRESS is a simple and versatile localization procedure that is readily adaptable to spectral relaxation time measurements by adding inversion or spin-echo refocusing pulses or to in vivo solvent-suppressed spectroscopy of proton (1H) metabolites using a combination of chemical-selective RF pulses. Also, the spatial information gathering efficiency of the technique can be improved to provide simultaneous acquisition of spectra from multiple volumes by interleaving excitation of adjacent planes within the normal relaxation recovery period. The spatial selectivity can be improved by adding additional selective excitation spin-echo refocusing pulses to achieve full, three-dimensional point resolved spectroscopy (PRESS) in a single excitation sequence. Alternatively, for samples with short spin-spin relaxation times, DRESS can be combined with other localization schemes, such as image-selected in vivo spectroscopy (ISIS), to provide complete gradient controlled three-dimensional localization with a reduced number of sequence cycles.

Original languageEnglish (US)
Pages (from-to)333-348
Number of pages16
JournalAnnals of the New York Academy of Sciences
Volume508
StatePublished - 1987
Externally publishedYes

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Nuclear magnetic resonance spectroscopy
Spectrum Analysis
Magnetic Resonance Spectroscopy
Magnetic Fields
Spectroscopy
Protons
Relaxation time
Nuclear magnetic resonance
Metabolites
Localization
Time measurement
Tissue
Magnetic fields
Recovery
Pulse

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Spatial localization in NMR spectroscopy in vivo. / Bottomley, Paul A.

In: Annals of the New York Academy of Sciences, Vol. 508, 1987, p. 333-348.

Research output: Contribution to journalArticle

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