Spatial control of adult stem cell fate using nanotopographic cues

Eun Hyun Ahn, Younghoon Kim, Kshitiz, Steven An, Junaid Afzal, Suengwon Lee, Moonkyu Kwak, Kahp Yang Suh, Deok Ho Kim, Andre Levchenko

Research output: Contribution to journalArticle

Abstract

Adult stem cells hold great promise as a source of diverse terminally differentiated cell types for tissue engineering applications. However, due to the complexity of chemical and mechanical cues specifying differentiation outcomes, development of arbitrarily complex geometric and structural arrangements of cells, adopting multiple fates from the same initial stem cell population, has been difficult. Here, we show that the topography of the cell adhesion substratum can be an instructive cue to adult stem cells and topographical variations can strongly bias the differentiation outcome of the cells towards adipocyte or osteocyte fates. Switches in cell fate decision from adipogenic to osteogenic lineages were accompanied by changes in cytoskeletal stiffness, spanning a considerable range in the cell softness/rigidity spectrum. Our findings suggest that human mesenchymal stem cells (hMSC) can respond to the varying density of nanotopographical cues by regulating their internal cytoskeletal network and use these mechanical changes to guide them toward making cell fate decisions. We used this finding to design a complex two-dimensional pattern of co-localized cells preferentially adopting two alternative fates, thus paving the road for designing and building more complex tissue constructs with diverse biomedical applications.

Original languageEnglish (US)
Pages (from-to)2401-2410
Number of pages10
JournalBiomaterials
Volume35
Issue number8
DOIs
StatePublished - Mar 2014

Fingerprint

Adult Stem Cells
Stem cells
Cues
Cell adhesion
Cell Engineering
Osteocytes
Tissue engineering
Rigidity
Topography
Tissue Engineering
Mesenchymal Stromal Cells
Adipocytes
Cell Adhesion
Switches
Stiffness
Tissue
Cell Differentiation
Stem Cells
Population

Keywords

  • Adipogenesis
  • Capillary force lithography
  • Differentiation
  • Human mesenchymal stem cells
  • Nanotopography
  • Osteogenesis

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Ahn, E. H., Kim, Y., Kshitiz, An, S., Afzal, J., Lee, S., ... Levchenko, A. (2014). Spatial control of adult stem cell fate using nanotopographic cues. Biomaterials, 35(8), 2401-2410. https://doi.org/10.1016/j.biomaterials.2013.11.037

Spatial control of adult stem cell fate using nanotopographic cues. / Ahn, Eun Hyun; Kim, Younghoon; Kshitiz, ; An, Steven; Afzal, Junaid; Lee, Suengwon; Kwak, Moonkyu; Suh, Kahp Yang; Kim, Deok Ho; Levchenko, Andre.

In: Biomaterials, Vol. 35, No. 8, 03.2014, p. 2401-2410.

Research output: Contribution to journalArticle

Ahn, EH, Kim, Y, Kshitiz, , An, S, Afzal, J, Lee, S, Kwak, M, Suh, KY, Kim, DH & Levchenko, A 2014, 'Spatial control of adult stem cell fate using nanotopographic cues', Biomaterials, vol. 35, no. 8, pp. 2401-2410. https://doi.org/10.1016/j.biomaterials.2013.11.037
Ahn, Eun Hyun ; Kim, Younghoon ; Kshitiz, ; An, Steven ; Afzal, Junaid ; Lee, Suengwon ; Kwak, Moonkyu ; Suh, Kahp Yang ; Kim, Deok Ho ; Levchenko, Andre. / Spatial control of adult stem cell fate using nanotopographic cues. In: Biomaterials. 2014 ; Vol. 35, No. 8. pp. 2401-2410.
@article{b4b2a9e01d0d4ea19cf5400e48a3b923,
title = "Spatial control of adult stem cell fate using nanotopographic cues",
abstract = "Adult stem cells hold great promise as a source of diverse terminally differentiated cell types for tissue engineering applications. However, due to the complexity of chemical and mechanical cues specifying differentiation outcomes, development of arbitrarily complex geometric and structural arrangements of cells, adopting multiple fates from the same initial stem cell population, has been difficult. Here, we show that the topography of the cell adhesion substratum can be an instructive cue to adult stem cells and topographical variations can strongly bias the differentiation outcome of the cells towards adipocyte or osteocyte fates. Switches in cell fate decision from adipogenic to osteogenic lineages were accompanied by changes in cytoskeletal stiffness, spanning a considerable range in the cell softness/rigidity spectrum. Our findings suggest that human mesenchymal stem cells (hMSC) can respond to the varying density of nanotopographical cues by regulating their internal cytoskeletal network and use these mechanical changes to guide them toward making cell fate decisions. We used this finding to design a complex two-dimensional pattern of co-localized cells preferentially adopting two alternative fates, thus paving the road for designing and building more complex tissue constructs with diverse biomedical applications.",
keywords = "Adipogenesis, Capillary force lithography, Differentiation, Human mesenchymal stem cells, Nanotopography, Osteogenesis",
author = "Ahn, {Eun Hyun} and Younghoon Kim and Kshitiz and Steven An and Junaid Afzal and Suengwon Lee and Moonkyu Kwak and Suh, {Kahp Yang} and Kim, {Deok Ho} and Andre Levchenko",
year = "2014",
month = "3",
doi = "10.1016/j.biomaterials.2013.11.037",
language = "English (US)",
volume = "35",
pages = "2401--2410",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "8",

}

TY - JOUR

T1 - Spatial control of adult stem cell fate using nanotopographic cues

AU - Ahn, Eun Hyun

AU - Kim, Younghoon

AU - Kshitiz,

AU - An, Steven

AU - Afzal, Junaid

AU - Lee, Suengwon

AU - Kwak, Moonkyu

AU - Suh, Kahp Yang

AU - Kim, Deok Ho

AU - Levchenko, Andre

PY - 2014/3

Y1 - 2014/3

N2 - Adult stem cells hold great promise as a source of diverse terminally differentiated cell types for tissue engineering applications. However, due to the complexity of chemical and mechanical cues specifying differentiation outcomes, development of arbitrarily complex geometric and structural arrangements of cells, adopting multiple fates from the same initial stem cell population, has been difficult. Here, we show that the topography of the cell adhesion substratum can be an instructive cue to adult stem cells and topographical variations can strongly bias the differentiation outcome of the cells towards adipocyte or osteocyte fates. Switches in cell fate decision from adipogenic to osteogenic lineages were accompanied by changes in cytoskeletal stiffness, spanning a considerable range in the cell softness/rigidity spectrum. Our findings suggest that human mesenchymal stem cells (hMSC) can respond to the varying density of nanotopographical cues by regulating their internal cytoskeletal network and use these mechanical changes to guide them toward making cell fate decisions. We used this finding to design a complex two-dimensional pattern of co-localized cells preferentially adopting two alternative fates, thus paving the road for designing and building more complex tissue constructs with diverse biomedical applications.

AB - Adult stem cells hold great promise as a source of diverse terminally differentiated cell types for tissue engineering applications. However, due to the complexity of chemical and mechanical cues specifying differentiation outcomes, development of arbitrarily complex geometric and structural arrangements of cells, adopting multiple fates from the same initial stem cell population, has been difficult. Here, we show that the topography of the cell adhesion substratum can be an instructive cue to adult stem cells and topographical variations can strongly bias the differentiation outcome of the cells towards adipocyte or osteocyte fates. Switches in cell fate decision from adipogenic to osteogenic lineages were accompanied by changes in cytoskeletal stiffness, spanning a considerable range in the cell softness/rigidity spectrum. Our findings suggest that human mesenchymal stem cells (hMSC) can respond to the varying density of nanotopographical cues by regulating their internal cytoskeletal network and use these mechanical changes to guide them toward making cell fate decisions. We used this finding to design a complex two-dimensional pattern of co-localized cells preferentially adopting two alternative fates, thus paving the road for designing and building more complex tissue constructs with diverse biomedical applications.

KW - Adipogenesis

KW - Capillary force lithography

KW - Differentiation

KW - Human mesenchymal stem cells

KW - Nanotopography

KW - Osteogenesis

UR - http://www.scopus.com/inward/record.url?scp=84891739845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891739845&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2013.11.037

DO - 10.1016/j.biomaterials.2013.11.037

M3 - Article

VL - 35

SP - 2401

EP - 2410

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 8

ER -