Soy consumption and histopathologic markers in breast tissue using tissue microarrays

Gertraud Maskarinec, Eva Oakkar, Martijn Verheus, Brenda Y. Hernandez, Jeffrey Killeen, Suzanne Cashin, J. Mark Cline

Research output: Contribution to journalArticle

Abstract

This study examined the relation of soy intake with hormonal and proliferation markers in benign and malignant breast tissue using tissue microarrays (TMAs). TMAs with up to 4 malignant and 4 benign tissue samples for 268 breast cancer cases were constructed. Soy intake in early life and in adulthood was assessed by questionnaire. The TMAs were stained for estrogen receptor (ER) , ER, progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2/neu), proliferating cell nuclear antigen (PCNA), and Ki-67 using standard immunohistochemical methods. Logistic regression was applied for statistical analysis. A higher percentage of women showed positive marker expression in malignant than in benign tissue. With one exception, HER2/neu, no significant associations between soy intake and pathologic markers were observed. Early life soy intake was associated with lower HER2/neu and PCNA staining of malignant tissue. In benign tissue, early life soy intake showed higher ER and PR expression, but no difference in proliferation markers. The results of this investigation provide some assurance that soy intake does not adversely affect markers of proliferation. TMAs were shown to be a useful tool for epidemiologic research.

Original languageEnglish (US)
Pages (from-to)708-716
Number of pages9
JournalNutrition and Cancer
Volume61
Issue number5
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Breast
Estrogen Receptors
Proliferating Cell Nuclear Antigen
Progesterone Receptors
Logistic Models
Staining and Labeling
Breast Neoplasms
Research

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Maskarinec, G., Oakkar, E., Verheus, M., Hernandez, B. Y., Killeen, J., Cashin, S., & Cline, J. M. (2009). Soy consumption and histopathologic markers in breast tissue using tissue microarrays. Nutrition and Cancer, 61(5), 708-716. https://doi.org/10.1080/01635580902913047

Soy consumption and histopathologic markers in breast tissue using tissue microarrays. / Maskarinec, Gertraud; Oakkar, Eva; Verheus, Martijn; Hernandez, Brenda Y.; Killeen, Jeffrey; Cashin, Suzanne; Cline, J. Mark.

In: Nutrition and Cancer, Vol. 61, No. 5, 09.2009, p. 708-716.

Research output: Contribution to journalArticle

Maskarinec, G, Oakkar, E, Verheus, M, Hernandez, BY, Killeen, J, Cashin, S & Cline, JM 2009, 'Soy consumption and histopathologic markers in breast tissue using tissue microarrays', Nutrition and Cancer, vol. 61, no. 5, pp. 708-716. https://doi.org/10.1080/01635580902913047
Maskarinec G, Oakkar E, Verheus M, Hernandez BY, Killeen J, Cashin S et al. Soy consumption and histopathologic markers in breast tissue using tissue microarrays. Nutrition and Cancer. 2009 Sep;61(5):708-716. https://doi.org/10.1080/01635580902913047
Maskarinec, Gertraud ; Oakkar, Eva ; Verheus, Martijn ; Hernandez, Brenda Y. ; Killeen, Jeffrey ; Cashin, Suzanne ; Cline, J. Mark. / Soy consumption and histopathologic markers in breast tissue using tissue microarrays. In: Nutrition and Cancer. 2009 ; Vol. 61, No. 5. pp. 708-716.
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