Sox11 reduces Caspase-6 cleavage and activity

Elaine Waldron-Roby, Janine Hoerauf, Nicolas Arbez, Shanshan Zhu, Kirsten Kulcsar, Christopher A Ross

Research output: Contribution to journalArticle

Abstract

The apoptotic cascade is an orchestrated event, whose final stages are mediated by effector caspases. Regulatory binding proteins have been identified for caspases such as cas-pase-3, -7, -8, and -9. Many of these proteins belong to the inhibitor of apoptosis (IAP) family. By contrast, caspase-6 is not believed to be influenced by IAPs, and little is known about its regulation. We therefore performed a yeast-two-hybrid screen using a constitutively inactive form of caspase-6 for bait in order to identify novel regulators of caspase-6 activity. Sox11 was identified as a potential caspase-6 interacting protein. Sox11 was capable of dramatically reducing caspase-6 activity, as well as preventing caspase-6 self-cleavage. Several regions, including amino acids 117-214 and 362-395 within sox11 as well as a nuclear localization signal (NLS) all contributed to the reduction in caspase-6 activity. Furthermore, sox11 was also capable of decreasing other effector caspase activity but not initiator caspases -8 and -9. The ability of sox11 to reduce effector caspase activity was also reflected in its capacity to reduce cell death following toxic insult. Interestingly, other sox proteins also had the ability to reduce caspase-6 activity but to a lesser extent than sox11.

Original languageEnglish (US)
Article number0141439
JournalPLoS One
Volume10
Issue number10
DOIs
StatePublished - Oct 27 2015

Fingerprint

caspase-6
Caspase 6
caspases
Effector Caspases
Initiator Caspases
caspase-8
Nuclear Localization Signals
nuclear localization signals
Proteins
proteins
Caspase 9
Caspase 8
Poisons
regulatory proteins
Cell death
Caspases
baits
Yeast
binding proteins
cell death

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sox11 reduces Caspase-6 cleavage and activity. / Waldron-Roby, Elaine; Hoerauf, Janine; Arbez, Nicolas; Zhu, Shanshan; Kulcsar, Kirsten; Ross, Christopher A.

In: PLoS One, Vol. 10, No. 10, 0141439, 27.10.2015.

Research output: Contribution to journalArticle

Waldron-Roby, Elaine ; Hoerauf, Janine ; Arbez, Nicolas ; Zhu, Shanshan ; Kulcsar, Kirsten ; Ross, Christopher A. / Sox11 reduces Caspase-6 cleavage and activity. In: PLoS One. 2015 ; Vol. 10, No. 10.
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