TY - JOUR
T1 - SOX10 distinguishes pilocytic and pilomyxoid astrocytomas from ependymomas but shows no differences in expression level in ependymomas from infants versus older children or among molecular subgroups
AU - Kleinschmidt-De Masters, B. K.
AU - Donson, Andrew M.
AU - Richmond, Abby M.
AU - Pekmezci, Melike
AU - Tihan, Tarik
AU - Foreman, Nicholas K.
N1 - Publisher Copyright:
© 2016 American Association of Neuropathologists, Inc. All rights reserved.
PY - 2016/4
Y1 - 2016/4
N2 - SOX10 is important in nonneoplastic oligodendroglial development, but mRNA transcripts and protein expression are identified in a wider variety of CNS glial neoplasms than oligodendrogliomas. We previously demonstrated high levels of SOX10 mRNA and protein in pilocytic astrocytomas (PAs) but not ependymomas (EPNs). We now extend these studies to investigate subsets of these 2 tumors that affect infants, pilomyxoid astrocytomas (PMAs) and infant (<1 year) ependymomas (iEPNs). By gene expression microarray analysis, we found that iEPNs and all EPNs in older children showed very low SOX10 expression levels, on average 7.1-fold below normal control tissues. EPN groups showed no significant difference in SOX10 expression between iEPN and EPN. PAs/PMAs had 24.1/29.4-fold higher transcript levels, respectively, than those in normal tissues. Using immunohistochemical analysis of adult, pediatric, and infantile EPNs and of PAs/PMAs, we found that EPNs from multiple anatomical locations and both age groups (n=228) never showed 3 diffuse nuclear immunostaining for SOX10; the majority were scored at 0 or 1. Conversely, almost all pediatric and adult PAs and PMAs (n=47) were scored as 3+. These results suggest that in select settings, SOX10 immunohistochemistry can supplement the diagnosis of PMA and PA and aid in distinguishing them from EPNs.
AB - SOX10 is important in nonneoplastic oligodendroglial development, but mRNA transcripts and protein expression are identified in a wider variety of CNS glial neoplasms than oligodendrogliomas. We previously demonstrated high levels of SOX10 mRNA and protein in pilocytic astrocytomas (PAs) but not ependymomas (EPNs). We now extend these studies to investigate subsets of these 2 tumors that affect infants, pilomyxoid astrocytomas (PMAs) and infant (<1 year) ependymomas (iEPNs). By gene expression microarray analysis, we found that iEPNs and all EPNs in older children showed very low SOX10 expression levels, on average 7.1-fold below normal control tissues. EPN groups showed no significant difference in SOX10 expression between iEPN and EPN. PAs/PMAs had 24.1/29.4-fold higher transcript levels, respectively, than those in normal tissues. Using immunohistochemical analysis of adult, pediatric, and infantile EPNs and of PAs/PMAs, we found that EPNs from multiple anatomical locations and both age groups (n=228) never showed 3 diffuse nuclear immunostaining for SOX10; the majority were scored at 0 or 1. Conversely, almost all pediatric and adult PAs and PMAs (n=47) were scored as 3+. These results suggest that in select settings, SOX10 immunohistochemistry can supplement the diagnosis of PMA and PA and aid in distinguishing them from EPNs.
KW - Developmental
KW - Gene expression microarray
KW - Infant ependymoma
KW - Myxopapillary ependymoma
KW - Pilocytic astrocytoma
KW - Pilomyxoid astrocytoma
KW - SOX10
UR - http://www.scopus.com/inward/record.url?scp=84961926648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961926648&partnerID=8YFLogxK
U2 - 10.1093/jnen/nlw010
DO - 10.1093/jnen/nlw010
M3 - Article
C2 - 26945037
AN - SCOPUS:84961926648
SN - 0022-3069
VL - 75
SP - 295
EP - 298
JO - Journal of neuropathology and experimental neurology
JF - Journal of neuropathology and experimental neurology
IS - 4
ER -