Sources of variability in repeated t-helper lymphocyte counts from human immunodeficiency virus type 1-infected patients: Total lymphocyte count fluctuations and diurnal cycle are important

Joseph L. Malone, Thomas E. Simms, Gregory C. Gray, Kenneth F. Wagner, J. Robert Burge, Donald S. Burke

Research output: Contribution to journalArticle

Abstract

The study objective was to determine the causes and magnitude of absolute CD4 (T4) count variation in human immunodeficiency virus type 1 (HlV-l)-infected (+) adult males. We conducted a prospective, blinded, and controlled study of 22 adult military male outpatients, including 16 HIV(+) [12 in Walter Reed stage (WR-) 1 through 5, 4 in WR-6 (AIDS)], and 6 HIV seronegative (—) healthy controls. Ten CD4+ cell counts were drawn within a 3-day interval from each patient at the following times: 0800, 1200, 1600, and 2200 h on day 1; and 0800, 1200, and 1600 h on days 2 and 3. A significant CD4+ cell count diurnal increase of 59 cells/mm3 was detected between 0800 h and 2200 h from the WR-1-5 patients (p = 0.018), although this diurnal change was significantly blunted (p = 0.028) as compared with the 506 cells/mm3 CD4+ cell count diurnal increase observed from the HIV(-) healthy controls. The coefficients of variation [CV = (standard deviation/average) x 100] of the three daily 0800 h CD4 cell counts from each patient were 15 (median) and 19 (average) for the WR-1-5 patient group. Blood leukocyte counts, differential fractions of lymphocytes, and total lymphocyte counts contributed more to the observed CD4+ cell count variability than did the CD4% measurements [CV = 7.5 (median), 11 (average)] obtained from flow cytometry. We conclude that the large fluctuations that we observed in repeated CD4+ cell counts in HIV(+) patients can be explained in part by CD4+ cell count diurnal cycle and in part by high variability in total lymphocyte counts. Awareness of the causes and expected magnitude of CD4+ cell count variation should facilitate the interpretation of CD4+ cell count results from individual HIV(+) and HIV-seronegative individuals.

Original languageEnglish (US)
Pages (from-to)144-151
Number of pages8
JournalJournal of Acquired Immune Deficiency Syndromes
Volume3
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Lymphocyte Count
CD4 Lymphocyte Count
HIV-1
HIV
Leukocyte Count
Flow Cytometry
Acquired Immunodeficiency Syndrome
Outpatients
Lymphocytes

Keywords

  • Blood cell count
  • CD4-lymphocytes
  • Circadian rhythm
  • Flow cytometry
  • Helper cells
  • HIV seropositivity
  • Periodicity

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Immunology and Allergy
  • Virology

Cite this

Sources of variability in repeated t-helper lymphocyte counts from human immunodeficiency virus type 1-infected patients : Total lymphocyte count fluctuations and diurnal cycle are important. / Malone, Joseph L.; Simms, Thomas E.; Gray, Gregory C.; Wagner, Kenneth F.; Burge, J. Robert; Burke, Donald S.

In: Journal of Acquired Immune Deficiency Syndromes, Vol. 3, No. 2, 1990, p. 144-151.

Research output: Contribution to journalArticle

Malone, Joseph L. ; Simms, Thomas E. ; Gray, Gregory C. ; Wagner, Kenneth F. ; Burge, J. Robert ; Burke, Donald S. / Sources of variability in repeated t-helper lymphocyte counts from human immunodeficiency virus type 1-infected patients : Total lymphocyte count fluctuations and diurnal cycle are important. In: Journal of Acquired Immune Deficiency Syndromes. 1990 ; Vol. 3, No. 2. pp. 144-151.
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abstract = "The study objective was to determine the causes and magnitude of absolute CD4 (T4) count variation in human immunodeficiency virus type 1 (HlV-l)-infected (+) adult males. We conducted a prospective, blinded, and controlled study of 22 adult military male outpatients, including 16 HIV(+) [12 in Walter Reed stage (WR-) 1 through 5, 4 in WR-6 (AIDS)], and 6 HIV seronegative (—) healthy controls. Ten CD4+ cell counts were drawn within a 3-day interval from each patient at the following times: 0800, 1200, 1600, and 2200 h on day 1; and 0800, 1200, and 1600 h on days 2 and 3. A significant CD4+ cell count diurnal increase of 59 cells/mm3 was detected between 0800 h and 2200 h from the WR-1-5 patients (p = 0.018), although this diurnal change was significantly blunted (p = 0.028) as compared with the 506 cells/mm3 CD4+ cell count diurnal increase observed from the HIV(-) healthy controls. The coefficients of variation [CV = (standard deviation/average) x 100] of the three daily 0800 h CD4 cell counts from each patient were 15 (median) and 19 (average) for the WR-1-5 patient group. Blood leukocyte counts, differential fractions of lymphocytes, and total lymphocyte counts contributed more to the observed CD4+ cell count variability than did the CD4{\%} measurements [CV = 7.5 (median), 11 (average)] obtained from flow cytometry. We conclude that the large fluctuations that we observed in repeated CD4+ cell counts in HIV(+) patients can be explained in part by CD4+ cell count diurnal cycle and in part by high variability in total lymphocyte counts. Awareness of the causes and expected magnitude of CD4+ cell count variation should facilitate the interpretation of CD4+ cell count results from individual HIV(+) and HIV-seronegative individuals.",
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