Sources of human schwann cells and the influence of donor age

Philip J. Boyer, Gerald F. Tuite, Robert C. Dauser, Karin M. Muraszko, Gihan I. Tennekoon, J. Lynn Rutkowski

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated several tissues as possible sources for culturing human Schwann cells. The average cell yield (total cell number/mg of nerve fascicle) obtained from adult autopsy cases and transplant organ donors was similar (2 × 104 and 2.9 × 104, respectively), but significantly higher yields were obtained from dorsal roots of pediatric patients undergoing selective dorsal rhizotomy (6.1 × 104). Fresh tissue was not essential since cells isolated from 0 to 20 h postmortem were equally viable. However, we found evidence that donor age affects the intrinsic growth rate of Schwann cells and perineurial fibroblasts in culture.

Original languageEnglish (US)
Pages (from-to)53-55
Number of pages3
JournalExperimental Neurology
Volume130
Issue number1
DOIs
StatePublished - Nov 1994
Externally publishedYes

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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