Somatic mutations reveal asymmetric cellular dynamics in the early human embryo

Young Seok Ju, Inigo Martincorena, Moritz Gerstung, Mia Petljak, Ludmil B. Alexandrov, Raheleh Rahbari, David C. Wedge, Helen R. Davies, Manasa Ramakrishna, Anthony Fullam, Sancha Martin, Christopher Alder, Nikita Patel, Steve Gamble, Sarah O'Meara, DIlip D. Giri, Torril Sauer, Sarah E. Pinder, Colin A. Purdie, Åke Borg & 21 others Henk Stunnenberg, Marc Van De Vijver, Benita K.T. Tan, Carlos Caldas, Andrew Tutt, Naoto T. Ueno, Laura J. Van 'T Veer, John W.M. Martens, Christos Sotiriou, Stian Knappskog, Paul N. Span, Sunil R. Lakhani, Jórunn Erla Eyfjörd, Anne Lise Børresen-Dale, Andrea Richardson, Alastair M. Thompson, Alain Viari, Matthew E. Hurles, Serena Nik-Zainal, Peter J. Campbell, Michael R. Stratton

Research output: Contribution to journalArticle

Abstract

Somatic cells acquire mutations throughout the course of an individual's life. Mutations occurring early in embryogenesis are often present in a substantial proportion of, but not all, cells in postnatal humans and thus have particular characteristics and effects. Depending on their location in the genome and the proportion of cells they are present in, these mosaic mutations can cause a wide range of genetic disease syndromes and predispose carriers to cancer. They have a high chance of being transmitted to offspring as de novo germline mutations and, in principle, can provide insights into early human embryonic cell lineages and their contributions to adult tissues. Although it is known that gross chromosomal abnormalities are remarkably common in early human embryos, our understanding of early embryonic somatic mutations is very limited. Here we use whole-genome sequences of normal blood from 241 adults to identify 163 early embryonic mutations. We estimate that approximately three base substitution mutations occur per cell per cell-doubling event in early human embryogenesis and these are mainly attributable to two known mutational signatures. We used the mutations to reconstruct developmental lineages of adult cells and demonstrate that the two daughter cells of many early embryonic cell-doubling events contribute asymmetrically to adult blood at an approximately 2:1 ratio. This study therefore provides insights into the mutation rates, mutational processes and developmental outcomes of cell dynamics that operate during early human embryogenesis.

Original languageEnglish (US)
Pages (from-to)714-718
Number of pages5
JournalNature
Volume543
Issue number7647
DOIs
StatePublished - Mar 30 2017
Externally publishedYes

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Embryonic Structures
Mutation
Embryonic Development
Genome
Inborn Genetic Diseases
Germ-Line Mutation
Cell Lineage
Mutation Rate
Chromosome Aberrations
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Ju, Y. S., Martincorena, I., Gerstung, M., Petljak, M., Alexandrov, L. B., Rahbari, R., ... Stratton, M. R. (2017). Somatic mutations reveal asymmetric cellular dynamics in the early human embryo. Nature, 543(7647), 714-718. https://doi.org/10.1038/nature21703

Somatic mutations reveal asymmetric cellular dynamics in the early human embryo. / Ju, Young Seok; Martincorena, Inigo; Gerstung, Moritz; Petljak, Mia; Alexandrov, Ludmil B.; Rahbari, Raheleh; Wedge, David C.; Davies, Helen R.; Ramakrishna, Manasa; Fullam, Anthony; Martin, Sancha; Alder, Christopher; Patel, Nikita; Gamble, Steve; O'Meara, Sarah; Giri, DIlip D.; Sauer, Torril; Pinder, Sarah E.; Purdie, Colin A.; Borg, Åke; Stunnenberg, Henk; Van De Vijver, Marc; Tan, Benita K.T.; Caldas, Carlos; Tutt, Andrew; Ueno, Naoto T.; Van 'T Veer, Laura J.; Martens, John W.M.; Sotiriou, Christos; Knappskog, Stian; Span, Paul N.; Lakhani, Sunil R.; Eyfjörd, Jórunn Erla; Børresen-Dale, Anne Lise; Richardson, Andrea; Thompson, Alastair M.; Viari, Alain; Hurles, Matthew E.; Nik-Zainal, Serena; Campbell, Peter J.; Stratton, Michael R.

In: Nature, Vol. 543, No. 7647, 30.03.2017, p. 714-718.

Research output: Contribution to journalArticle

Ju, YS, Martincorena, I, Gerstung, M, Petljak, M, Alexandrov, LB, Rahbari, R, Wedge, DC, Davies, HR, Ramakrishna, M, Fullam, A, Martin, S, Alder, C, Patel, N, Gamble, S, O'Meara, S, Giri, DID, Sauer, T, Pinder, SE, Purdie, CA, Borg, Å, Stunnenberg, H, Van De Vijver, M, Tan, BKT, Caldas, C, Tutt, A, Ueno, NT, Van 'T Veer, LJ, Martens, JWM, Sotiriou, C, Knappskog, S, Span, PN, Lakhani, SR, Eyfjörd, JE, Børresen-Dale, AL, Richardson, A, Thompson, AM, Viari, A, Hurles, ME, Nik-Zainal, S, Campbell, PJ & Stratton, MR 2017, 'Somatic mutations reveal asymmetric cellular dynamics in the early human embryo', Nature, vol. 543, no. 7647, pp. 714-718. https://doi.org/10.1038/nature21703
Ju YS, Martincorena I, Gerstung M, Petljak M, Alexandrov LB, Rahbari R et al. Somatic mutations reveal asymmetric cellular dynamics in the early human embryo. Nature. 2017 Mar 30;543(7647):714-718. https://doi.org/10.1038/nature21703
Ju, Young Seok ; Martincorena, Inigo ; Gerstung, Moritz ; Petljak, Mia ; Alexandrov, Ludmil B. ; Rahbari, Raheleh ; Wedge, David C. ; Davies, Helen R. ; Ramakrishna, Manasa ; Fullam, Anthony ; Martin, Sancha ; Alder, Christopher ; Patel, Nikita ; Gamble, Steve ; O'Meara, Sarah ; Giri, DIlip D. ; Sauer, Torril ; Pinder, Sarah E. ; Purdie, Colin A. ; Borg, Åke ; Stunnenberg, Henk ; Van De Vijver, Marc ; Tan, Benita K.T. ; Caldas, Carlos ; Tutt, Andrew ; Ueno, Naoto T. ; Van 'T Veer, Laura J. ; Martens, John W.M. ; Sotiriou, Christos ; Knappskog, Stian ; Span, Paul N. ; Lakhani, Sunil R. ; Eyfjörd, Jórunn Erla ; Børresen-Dale, Anne Lise ; Richardson, Andrea ; Thompson, Alastair M. ; Viari, Alain ; Hurles, Matthew E. ; Nik-Zainal, Serena ; Campbell, Peter J. ; Stratton, Michael R. / Somatic mutations reveal asymmetric cellular dynamics in the early human embryo. In: Nature. 2017 ; Vol. 543, No. 7647. pp. 714-718.
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T1 - Somatic mutations reveal asymmetric cellular dynamics in the early human embryo

AU - Ju, Young Seok

AU - Martincorena, Inigo

AU - Gerstung, Moritz

AU - Petljak, Mia

AU - Alexandrov, Ludmil B.

AU - Rahbari, Raheleh

AU - Wedge, David C.

AU - Davies, Helen R.

AU - Ramakrishna, Manasa

AU - Fullam, Anthony

AU - Martin, Sancha

AU - Alder, Christopher

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AU - O'Meara, Sarah

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AU - Purdie, Colin A.

AU - Borg, Åke

AU - Stunnenberg, Henk

AU - Van De Vijver, Marc

AU - Tan, Benita K.T.

AU - Caldas, Carlos

AU - Tutt, Andrew

AU - Ueno, Naoto T.

AU - Van 'T Veer, Laura J.

AU - Martens, John W.M.

AU - Sotiriou, Christos

AU - Knappskog, Stian

AU - Span, Paul N.

AU - Lakhani, Sunil R.

AU - Eyfjörd, Jórunn Erla

AU - Børresen-Dale, Anne Lise

AU - Richardson, Andrea

AU - Thompson, Alastair M.

AU - Viari, Alain

AU - Hurles, Matthew E.

AU - Nik-Zainal, Serena

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