Somatic mutations of PTEN in glioblastoma multiforme

Steven I. Wang, Janusz Puc, Jing Li, Jeffrey N. Bruce, Paul Cairns, David Sidransky, Ramon Parsons

Research output: Contribution to journalArticlepeer-review

Abstract

Alterations of the PTEN gene occur in glioblastoma multiforme. To determine the frequency of PTEN alteration, 34 consecutive glioblastomas were studied in detail. Sequencing each of the nine exons amplified from tumor DNA revealed 11 mutations. Analysis of polymorphic markers within and surrounding the PTEN gene identified an additional four homozygous deletion mutations. Loss of heterozygosity (LOH) was observed in 25 of 34 (74%) cases. All mutations occurred in the presence of LOH. PTEN was mutated in 44% (15 of 34) of all glioblastomas studied and 60% (15 of 25) of tumors with LOH on 10q. Thus, PTEN appears to be the major target of inactivation on chromosome 10q in glioblastoma multiforme.

Original languageEnglish (US)
Pages (from-to)4183-4186
Number of pages4
JournalCancer Research
Volume57
Issue number19
StatePublished - Oct 1 1997

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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