Somatic embryogenesis from three commercially important inbreds of Zea mays

J. W. Fahey, J. N. Reed, T. L. Readdy, G. M. Pace

Research output: Contribution to journalArticle

Abstract

Zea mays (maize) genotypes B73, Mo17 and LH38 were evaluated for their capacity to undergo somatic embryogenesis. Over 1500 immature embryos (ie's) of B73, 2900 ie's of LH38 and 400 ie's of Mo17 were excised 10-17 days after pollination and plated on six different media. Overall response, reported as a percentage of the ie's plated that developed embryogenic callus, was 2.1%, 1.6% and 26% for LH38, B73 and Mo17, respectively. Best response on a given medium for each of these genotypes was 9.2% (LH38), 4.4% (B73) and 100% (Mo17). Other parameters examined for their effects on production of embryogenic callus included self vs. sib pollination, ear ranking (1st, 2nd or 3rd ear), and temperature shock, all of which had no significant effect. Plantlets regenerated from selected treatments of B73 have been grown to maturity, selfed or sibbed and seed collected for field evaluation.

Original languageEnglish (US)
Pages (from-to)35-38
Number of pages4
JournalPlant Cell Reports
Volume5
Issue number1
DOIs
StatePublished - Feb 1 1986
Externally publishedYes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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