Solubilized adenosine receptors in the brain: Regulation by guanine nucleotides

Moshe Gavish; Robert R. Goodman; Solomon H. Snyder

doi:10.1126/science.6280275

Solubilized adenosine receptors in the brain: Regulation by guanine nucleotides

Moshe Gavish, Robert R. Goodman, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Adenosine receptors associated with a reduction of adenylate cyclase and labeled by tritium-labeled cyclohexyladenosine can be solubilized from brain membranes with sodium chelate. Regulation of receptor binding by guanine nucleotides is retained in the soluble state. Influences of cations observed in membrane preparations of adenosine receptors are no longer detected with the solubilized receptors. The apparent retention of a complex of receptors and guanosine triphosphate binding but not cation binding protein in the soluble state may permit a molecular analysis of receptor regulation.

Original language	English (US)
Pages (from-to)	1633-1635
Number of pages	3
Journal	Science
Volume	216
Issue number	4540
DOIs	https://doi.org/10.1126/science.6280275
State	Published - 1982

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AB - Adenosine receptors associated with a reduction of adenylate cyclase and labeled by tritium-labeled cyclohexyladenosine can be solubilized from brain membranes with sodium chelate. Regulation of receptor binding by guanine nucleotides is retained in the soluble state. Influences of cations observed in membrane preparations of adenosine receptors are no longer detected with the solubilized receptors. The apparent retention of a complex of receptors and guanosine triphosphate binding but not cation binding protein in the soluble state may permit a molecular analysis of receptor regulation.

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Solubilized adenosine receptors in the brain: Regulation by guanine nucleotides

Abstract

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