Solubilization and anionic regulation of cerebral sedative/convulsant receptors labeled with [35S] tert-butylbicyclophosphorothionate (TBPS)

Rosario R. Trifiletti, Adele M. Snowman, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

Binding activity for the cage convulsant [35S]-tert-butylbicyclophosphorothionate, which appears to label a site closely associated with the chloride ionophore of the GABAA/benzodiazepine receptor complex has been solubilized from rat cerebral cortex using the zwitterionic detergent CHAPS. Of several detergents screened, only CHAPS and CHAPSO were capable of solubilizing the binding activity with good recovery. The pharmacologic specificity of soluble [35S]-tert-butylbicyclophosphorothionate binding is very similar to the membrane state. In both the membrane and soluble state, [35S]-tert-butylbicyclophosphorothionate binding is enhanced by anions which support inhibitory post-synaptic potentials ("Eccles anions"), suggesting that [35S]-t-butylbicyclophosphorothionate may label chloride channels thought to be involved in these potentials. Since this solubilization procedure also preserves GABA and benzodiazepine binding and their regulation by drugs such as barbiturates, purification and isolation of the macromolecular complex including chloride channel and GABA-benzodiazepine sites may be feasible.

Original languageEnglish (US)
Pages (from-to)692-699
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume120
Issue number2
DOIs
StatePublished - Apr 30 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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