Solid state dna sizing by atomic force microscopy

Y. Fang, T. Spisz, N. P D Costa, Roger H Reeves, I. N. Bankman

Research output: Contribution to journalArticle

Abstract

Sizing DNA molecules is our of the most widely used analytiial approaches in molecular biology and biochemistry. Although several alternate approaches have been proposed, most of DNA sizing is performed by gel elect rophoresi-,. Alotnic force microscopy (AKM) allows rapid, accurate and reproducible visialization ol DNA molecules adsorbed onto solid substrate. Mere we propose a solid state DNA si/ing method based on ATM. Substrates suitable for rapid and easy immobilization of DNA, and subsequent ATM imaging have been developed. DNA images are subjected to automatic length determination us ing pattern-recognition software. At present when processing a single sample, sample preparation, AKM imaging and analysis takes l-ri-2U minutes. However, we experte that the average sizing time will eventually be less than 60 seconds. Currently solid st ate DNA sizing yields results comparable to agarose gel elect rophoresis for many types of samples. Decoration of DNA fragments by inactive restriction enzymes or other sequence specific binding/modifications should provide important information in addition to the size.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

Fingerprint

atomic force microscopy
Atomic Force Microscopy
Atomic force microscopy
DNA
Automatic teller machines
Gels
gels
image analysis
Imaging techniques
Biochemistry
Molecular biology
Molecules
Substrates
sampling
Immobilization
biochemistry
Sepharose
molecular biology
agarose
Pattern recognition

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Fang, Y., Spisz, T., Costa, N. P. D., Reeves, R. H., & Bankman, I. N. (1997). Solid state dna sizing by atomic force microscopy. FASEB Journal, 11(9).

Solid state dna sizing by atomic force microscopy. / Fang, Y.; Spisz, T.; Costa, N. P D; Reeves, Roger H; Bankman, I. N.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Fang, Y, Spisz, T, Costa, NPD, Reeves, RH & Bankman, IN 1997, 'Solid state dna sizing by atomic force microscopy', FASEB Journal, vol. 11, no. 9.
Fang Y, Spisz T, Costa NPD, Reeves RH, Bankman IN. Solid state dna sizing by atomic force microscopy. FASEB Journal. 1997;11(9).
Fang, Y. ; Spisz, T. ; Costa, N. P D ; Reeves, Roger H ; Bankman, I. N. / Solid state dna sizing by atomic force microscopy. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
@article{d3820810e40b4d2ba2cd519f304f749b,
title = "Solid state dna sizing by atomic force microscopy",
abstract = "Sizing DNA molecules is our of the most widely used analytiial approaches in molecular biology and biochemistry. Although several alternate approaches have been proposed, most of DNA sizing is performed by gel elect rophoresi-,. Alotnic force microscopy (AKM) allows rapid, accurate and reproducible visialization ol DNA molecules adsorbed onto solid substrate. Mere we propose a solid state DNA si/ing method based on ATM. Substrates suitable for rapid and easy immobilization of DNA, and subsequent ATM imaging have been developed. DNA images are subjected to automatic length determination us ing pattern-recognition software. At present when processing a single sample, sample preparation, AKM imaging and analysis takes l-ri-2U minutes. However, we experte that the average sizing time will eventually be less than 60 seconds. Currently solid st ate DNA sizing yields results comparable to agarose gel elect rophoresis for many types of samples. Decoration of DNA fragments by inactive restriction enzymes or other sequence specific binding/modifications should provide important information in addition to the size.",
author = "Y. Fang and T. Spisz and Costa, {N. P D} and Reeves, {Roger H} and Bankman, {I. N.}",
year = "1997",
language = "English (US)",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "9",

}

TY - JOUR

T1 - Solid state dna sizing by atomic force microscopy

AU - Fang, Y.

AU - Spisz, T.

AU - Costa, N. P D

AU - Reeves, Roger H

AU - Bankman, I. N.

PY - 1997

Y1 - 1997

N2 - Sizing DNA molecules is our of the most widely used analytiial approaches in molecular biology and biochemistry. Although several alternate approaches have been proposed, most of DNA sizing is performed by gel elect rophoresi-,. Alotnic force microscopy (AKM) allows rapid, accurate and reproducible visialization ol DNA molecules adsorbed onto solid substrate. Mere we propose a solid state DNA si/ing method based on ATM. Substrates suitable for rapid and easy immobilization of DNA, and subsequent ATM imaging have been developed. DNA images are subjected to automatic length determination us ing pattern-recognition software. At present when processing a single sample, sample preparation, AKM imaging and analysis takes l-ri-2U minutes. However, we experte that the average sizing time will eventually be less than 60 seconds. Currently solid st ate DNA sizing yields results comparable to agarose gel elect rophoresis for many types of samples. Decoration of DNA fragments by inactive restriction enzymes or other sequence specific binding/modifications should provide important information in addition to the size.

AB - Sizing DNA molecules is our of the most widely used analytiial approaches in molecular biology and biochemistry. Although several alternate approaches have been proposed, most of DNA sizing is performed by gel elect rophoresi-,. Alotnic force microscopy (AKM) allows rapid, accurate and reproducible visialization ol DNA molecules adsorbed onto solid substrate. Mere we propose a solid state DNA si/ing method based on ATM. Substrates suitable for rapid and easy immobilization of DNA, and subsequent ATM imaging have been developed. DNA images are subjected to automatic length determination us ing pattern-recognition software. At present when processing a single sample, sample preparation, AKM imaging and analysis takes l-ri-2U minutes. However, we experte that the average sizing time will eventually be less than 60 seconds. Currently solid st ate DNA sizing yields results comparable to agarose gel elect rophoresis for many types of samples. Decoration of DNA fragments by inactive restriction enzymes or other sequence specific binding/modifications should provide important information in addition to the size.

UR - http://www.scopus.com/inward/record.url?scp=33750147209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750147209&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750147209

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 9

ER -