Solid-phase sequence scanning for drug resistance detection in tuberculosis

S. R. Head, K. Parikh, Y. H. Rogers, William Ramses Bishai, Philip Goelet, M. T. Boyce-Jacino

Research output: Contribution to journalArticle

Abstract

DNA chip arrays hold considerable promise for diagnostic sequencing of polymerase chain reaction (PCR) products. To date, however, arrays have been relatively expensive, complex to use and difficult to interpret, preventing their adaptation to the clinical lab. A moderate density array method has been developed that enables efficient, easy-to-interpret and robust solid-phase PCR product sequencing. Here, the results of Mycobacterium tuberculosis rifampin resistance mutation detection by primer-extension-based sequence scanning of the rpoB gene of M. tuberculosis are presented. Rifampin resistant clinical isolates were identified in as little as 1 h post PCR amplification with visual results detection.

Original languageEnglish (US)
Pages (from-to)81-87
Number of pages7
JournalMolecular and Cellular Probes
Volume13
Issue number2
DOIs
StatePublished - Apr 1999

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Drug Resistance
Tuberculosis
Rifampin
Oligonucleotide Array Sequence Analysis
Mycobacterium tuberculosis
Polymerase Chain Reaction
Mutation
Genes

Keywords

  • DNA arrays
  • Drug resistance
  • GBA
  • Mycobacterium tuberculosis
  • Primer extension
  • Solid-phase

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Solid-phase sequence scanning for drug resistance detection in tuberculosis. / Head, S. R.; Parikh, K.; Rogers, Y. H.; Bishai, William Ramses; Goelet, Philip; Boyce-Jacino, M. T.

In: Molecular and Cellular Probes, Vol. 13, No. 2, 04.1999, p. 81-87.

Research output: Contribution to journalArticle

Head, S. R. ; Parikh, K. ; Rogers, Y. H. ; Bishai, William Ramses ; Goelet, Philip ; Boyce-Jacino, M. T. / Solid-phase sequence scanning for drug resistance detection in tuberculosis. In: Molecular and Cellular Probes. 1999 ; Vol. 13, No. 2. pp. 81-87.
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