Abstract
DNA chip arrays hold considerable promise for diagnostic sequencing of polymerase chain reaction (PCR) products. To date, however, arrays have been relatively expensive, complex to use and difficult to interpret, preventing their adaptation to the clinical lab. A moderate density array method has been developed that enables efficient, easy-to-interpret and robust solid-phase PCR product sequencing. Here, the results of Mycobacterium tuberculosis rifampin resistance mutation detection by primer-extension-based sequence scanning of the rpoB gene of M. tuberculosis are presented. Rifampin resistant clinical isolates were identified in as little as 1 h post PCR amplification with visual results detection.
Original language | English (US) |
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Pages (from-to) | 81-87 |
Number of pages | 7 |
Journal | Molecular and Cellular Probes |
Volume | 13 |
Issue number | 2 |
DOIs | |
State | Published - Apr 1999 |
Keywords
- DNA arrays
- Drug resistance
- GBA
- Mycobacterium tuberculosis
- Primer extension
- Solid-phase
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology