Solid-phase sequence scanning for drug resistance detection in tuberculosis

S. R. Head, K. Parikh, Y. H. Rogers, W. Bishai, P. Goelet, M. T. Boyce-Jacino

Research output: Contribution to journalArticlepeer-review

Abstract

DNA chip arrays hold considerable promise for diagnostic sequencing of polymerase chain reaction (PCR) products. To date, however, arrays have been relatively expensive, complex to use and difficult to interpret, preventing their adaptation to the clinical lab. A moderate density array method has been developed that enables efficient, easy-to-interpret and robust solid-phase PCR product sequencing. Here, the results of Mycobacterium tuberculosis rifampin resistance mutation detection by primer-extension-based sequence scanning of the rpoB gene of M. tuberculosis are presented. Rifampin resistant clinical isolates were identified in as little as 1 h post PCR amplification with visual results detection.

Original languageEnglish (US)
Pages (from-to)81-87
Number of pages7
JournalMolecular and Cellular Probes
Volume13
Issue number2
DOIs
StatePublished - Apr 1999

Keywords

  • DNA arrays
  • Drug resistance
  • GBA
  • Mycobacterium tuberculosis
  • Primer extension
  • Solid-phase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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