Solid lipid nanoparticles (SLNs) for intracellular targeting applications

Xiomara Calderón-Colón, Giorgio Raimondi, Jason J. Benkoski, Julia B. Patrone

Research output: Contribution to journalArticle

Abstract

Nanoparticle-based delivery vehicles have shown great promise for intracellular targeting applications, providing a mechanism to specifically alter cellular signaling and gene expression. In a previous investigation, the synthesis of ultra-small solid lipid nanoparticles (SLNs) for topical drug delivery and biomarker detection applications was demonstrated. SLNs are a well-studied example of a nanoparticle delivery system that has emerged as a promising drug delivery vehicle. In this study, SLNs were loaded with a fluorescent dye and used as a model to investigate particle-cell interactions. The phase inversion temperature (PIT) method was used for the synthesis of ultra-small populations of biocompatible nanoparticles. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylphenyltetrazolium bromide (MTT) assay was utilized in order to establish appropriate dosing levels prior to the nanoparticle-cell interaction studies. Furthermore, primary human dermal fibroblasts and mouse dendritic cells were exposed to dye-loaded SLN over time and the interactions with respect to toxicity and particle uptake were characterized using fluorescence microscopy and flow cytometry. This study demonstrated that ultra-small SLNs, as a nanoparticle delivery system, are suitable for intracellular targeting of different cell types.

Original languageEnglish (US)
Article numbere53102
JournalJournal of Visualized Experiments
Volume2015
Issue number105
DOIs
StatePublished - Nov 17 2015

Keywords

  • Bioengineering
  • Dendritic cells
  • Fibroblasts
  • Intracellular targeting
  • Issue 105
  • Nanobiotechnology
  • Solid lipid nanoparticles

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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