Software-automated counting of Ki-67 proliferation index correlates with pathologic grade and disease progression of follicular lymphomas

Mark A. Samols, Nathan E. Smith, Jonathan M. Gerber, Milena Vuica-Ross, Christopher Gocke, Kathleen Burns, Michael J Borowitz, Toby C. Cornish, Amy S Duffield

Research output: Contribution to journalArticle

Abstract

Objectives: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL). Methods: High-power field equivalents were extracted from H&E-and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement. Results: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade. Conclusions: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.

Original languageEnglish (US)
Pages (from-to)579-587
Number of pages9
JournalAmerican Journal of Clinical Pathology
Volume140
Issue number4
DOIs
StatePublished - Oct 2013

Fingerprint

Follicular Lymphoma
Disease Progression
Software
Radiation
Drug Therapy

Keywords

  • Automated counting
  • Follicular lymphoma
  • Histologic grade
  • Ki-67
  • Proliferation index
  • Quantitation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Software-automated counting of Ki-67 proliferation index correlates with pathologic grade and disease progression of follicular lymphomas. / Samols, Mark A.; Smith, Nathan E.; Gerber, Jonathan M.; Vuica-Ross, Milena; Gocke, Christopher; Burns, Kathleen; Borowitz, Michael J; Cornish, Toby C.; Duffield, Amy S.

In: American Journal of Clinical Pathology, Vol. 140, No. 4, 10.2013, p. 579-587.

Research output: Contribution to journalArticle

@article{dc2e5a0ba81847e19c752f6bd749bd5e,
title = "Software-automated counting of Ki-67 proliferation index correlates with pathologic grade and disease progression of follicular lymphomas",
abstract = "Objectives: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL). Methods: High-power field equivalents were extracted from H&E-and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement. Results: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade. Conclusions: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.",
keywords = "Automated counting, Follicular lymphoma, Histologic grade, Ki-67, Proliferation index, Quantitation",
author = "Samols, {Mark A.} and Smith, {Nathan E.} and Gerber, {Jonathan M.} and Milena Vuica-Ross and Christopher Gocke and Kathleen Burns and Borowitz, {Michael J} and Cornish, {Toby C.} and Duffield, {Amy S}",
year = "2013",
month = "10",
doi = "10.1309/AJCPTMA1F6LWYTQV",
language = "English (US)",
volume = "140",
pages = "579--587",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "4",

}

TY - JOUR

T1 - Software-automated counting of Ki-67 proliferation index correlates with pathologic grade and disease progression of follicular lymphomas

AU - Samols, Mark A.

AU - Smith, Nathan E.

AU - Gerber, Jonathan M.

AU - Vuica-Ross, Milena

AU - Gocke, Christopher

AU - Burns, Kathleen

AU - Borowitz, Michael J

AU - Cornish, Toby C.

AU - Duffield, Amy S

PY - 2013/10

Y1 - 2013/10

N2 - Objectives: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL). Methods: High-power field equivalents were extracted from H&E-and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement. Results: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade. Conclusions: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.

AB - Objectives: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL). Methods: High-power field equivalents were extracted from H&E-and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement. Results: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade. Conclusions: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.

KW - Automated counting

KW - Follicular lymphoma

KW - Histologic grade

KW - Ki-67

KW - Proliferation index

KW - Quantitation

UR - http://www.scopus.com/inward/record.url?scp=84884919123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884919123&partnerID=8YFLogxK

U2 - 10.1309/AJCPTMA1F6LWYTQV

DO - 10.1309/AJCPTMA1F6LWYTQV

M3 - Article

C2 - 24045557

AN - SCOPUS:84884919123

VL - 140

SP - 579

EP - 587

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 4

ER -