Abstract
Objectives: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL). Methods: High-power field equivalents were extracted from H&E-and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement. Results: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade. Conclusions: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 579-587 |
| Number of pages | 9 |
| Journal | American Journal of Clinical Pathology |
| Volume | 140 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 2013 |
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Keywords
- Automated counting
- Follicular lymphoma
- Histologic grade
- Ki-67
- Proliferation index
- Quantitation
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Software-automated counting of Ki-67 proliferation index correlates with pathologic grade and disease progression of follicular lymphomas. / Samols, Mark A.; Smith, Nathan E.; Gerber, Jonathan M.; Vuica-Ross, Milena; Gocke, Christopher; Burns, Kathleen; Borowitz, Michael J; Cornish, Toby C.; Duffield, Amy S.
In: American Journal of Clinical Pathology, Vol. 140, No. 4, 10.2013, p. 579-587.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Software-automated counting of Ki-67 proliferation index correlates with pathologic grade and disease progression of follicular lymphomas
AU - Samols, Mark A.
AU - Smith, Nathan E.
AU - Gerber, Jonathan M.
AU - Vuica-Ross, Milena
AU - Gocke, Christopher
AU - Burns, Kathleen
AU - Borowitz, Michael J
AU - Cornish, Toby C.
AU - Duffield, Amy S
PY - 2013/10
Y1 - 2013/10
N2 - Objectives: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL). Methods: High-power field equivalents were extracted from H&E-and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement. Results: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade. Conclusions: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.
AB - Objectives: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL). Methods: High-power field equivalents were extracted from H&E-and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement. Results: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade. Conclusions: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.
KW - Automated counting
KW - Follicular lymphoma
KW - Histologic grade
KW - Ki-67
KW - Proliferation index
KW - Quantitation
UR - http://www.scopus.com/inward/record.url?scp=84884919123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884919123&partnerID=8YFLogxK
U2 - 10.1309/AJCPTMA1F6LWYTQV
DO - 10.1309/AJCPTMA1F6LWYTQV
M3 - Article
C2 - 24045557
AN - SCOPUS:84884919123
VL - 140
SP - 579
EP - 587
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -