Sodium‐dependent calcium extrusion and sensitivity regulation in retinal cones of the salamander.

K. Nakatani, K. W. Yau

Research output: Contribution to journalArticlepeer-review

Abstract

1. Membrane current was recorded from an isolated, dark‐adapted salamander cone by sucking its inner segment into a tight‐fitting glass pipette containing Ringer solution. The outer segment of the cell was exposed to a bath solution that could be changed rapidly. 2. After removing Na+ from the bath Ringer solution for a short period of time in darkness (the ‘loading period’), a transient inward current was observed upon restoring it in bright light. A similar but longer‐lasting current was observed when Na+ was restored in the light after a large Ca2+ influx was induced through the light‐sensitive conductance in darkness. 3. The above transient current was not observed if Li+ or guanidinium was substituted for Na+ in the light, or if Ba2+ was substituted for Ca2+ during the dark loading period. However, a current was observed if Sr2+ was the substituting ion for Ca2+ during loading. These observations suggested that the current was associated with an electrogenic Na+‐dependent Ca2+ efflux at the cone outer segment. 4. The saturated amplitude of the exchange current was 12‐25 pA with a mean around 16 pA. This is very comparable to that measured in the outer segment of a salamander rod under similar conditions. 5. By comparing a known Ca2+ load in a cone outer segment to the subsequent charge transfer through the exchange, we estimated that the stoichiometry of the exchange was near 3Na+:1Ca2+. 6. With a small Ca2+ load, or in the presence of Cs+ around the inner segment, the final temporal decline of the Na+‐Ca2+ exchange current was roughly exponential, with a mean time constant of about 100 ms. This decline is about four times faster than that measured in rods. We interpret the shorter time constant in cones to reflect a faster rate of decline of intracellular free Ca2+ in their outer segments resulting from the exchange activity. 7. In the absence of external Na+, and hence any Na+‐dependent Ca2+ efflux, the absolute sensitivity of a cone to a dim flash was several times higher than in normal Ringer solution. 8. A roughly similar increase in light sensitivity was observed for a rod under the same conditions. 9. We conclude that the Na+‐dependent Ca2+ efflux, through lowering intracellular free Ca2+ in the light, has a role in regulating the absolute light sensitivity in cones as it does in rods.(ABSTRACT TRUNCATED AT 400 WORDS)

Original languageEnglish (US)
Pages (from-to)525-548
Number of pages24
JournalThe Journal of physiology
Volume409
Issue number1
DOIs
StatePublished - Feb 1 1989

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Sodium‐dependent calcium extrusion and sensitivity regulation in retinal cones of the salamander.'. Together they form a unique fingerprint.

Cite this