Sodium butyrate inhibits pathogenic yeast growth and enhances the functions of macrophages

Long Nam Nguyen, Livia Cristina Liporagi Lopes, Radames J.B. Cordero, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

Abstract

Objectives: Butyrate is a short-chain fatty acid that is produced by several human commensal bacteria, such as Clostridium and Lactobacillus species. Butyrate is also known to inhibit histone deacetylase. In this study we assessed the antifungal activity of sodium butyrate (SB) against the human pathogenic yeasts Candida albicans, Candida parapsilosis and Cryptococcus neoformans. Methods: The growth and virulence traits of the yeasts were assayed in vitro and during interaction with macrophages in the presence of SB. Results: SB strongly inhibited yeast growth in a concentration-dependent manner, inhibited virulence traits such as filamentation in C. albicans and melanization and capsule formation in C. neoformans and, importantly, significantly decreased yeast biofilm formation. SB also enhanced the antifungal activity of azole drugs. Notably, SB augmented the antifungal activity of macrophages by enhancing the production of reactive oxygen species. The phagocytic rate and killing activity of macrophages significantly increased in the presence of SB, which coincided with an increase in nitric oxide production. Conclusions: These results demonstrate that SB exerts significant antifungal activity on pathogenic yeasts and enhances the antimicrobial actions of macrophages in response to these microbes.

Original languageEnglish (US)
Article numberdkr358
Pages (from-to)2573-2580
Number of pages8
JournalJournal of Antimicrobial Chemotherapy
Volume66
Issue number11
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

Keywords

  • Candida species
  • Cryptococcus neoformans
  • Fungal virulence
  • Yeast infection

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

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