Sodium butyrate and a T lymphocyte cell line-derived differentiation factor induce basophilic differentiation of the human promyelocytic leukemia cell line HL-60

S. R. Hutt-Taylor, D. Harnish, M. Richardson, T. Ishizaka, J. A. Denburg

Research output: Contribution to journalArticle

Abstract

Sodium butyrate induces basophilic differentiation of HL-60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HL-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staining azurophilic with May-Grunwald-Giemsa and metachromatically with toluidine blue. The histamine content of induced HL-60 cells is 50 ng/106 cells with sodium butyrate alone or 190 ng/106 cells with butyrate in combination with Mo-CM. Induced cells release histamine in response to anti-IgE and have receptors for the Fc portion of human IgE. The basophilic cell-differentiating activity present in Mo-CM appears to be distinct from several other cytokines including recombinant human interleukin-1α, interleukin-2, interferon-γ, interferon-α, murine interleukin-3, erythroid-potentiating activity, and purified human granulocyte/macrophage colony-stimulating factor. This is the first demonstration of a cell line that is capable of differentiation along the basophil lineage and could provide a useful model for examining biochemical and molecular events associated with basophil differentiation.

Original languageEnglish (US)
Pages (from-to)209-215
Number of pages7
JournalBlood
Volume71
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

Butyric Acid
T-cells
Conditioned Culture Medium
Leukemia
Cells
T-Lymphocytes
Cell Line
Interferons
Histamine
Basophils
HL-60 Cells
Tolonium Chloride
Fc Receptors
Butyrates
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Immunoglobulin E
Interleukin-2
Cytoplasmic Granules

ASJC Scopus subject areas

  • Hematology

Cite this

Sodium butyrate and a T lymphocyte cell line-derived differentiation factor induce basophilic differentiation of the human promyelocytic leukemia cell line HL-60. / Hutt-Taylor, S. R.; Harnish, D.; Richardson, M.; Ishizaka, T.; Denburg, J. A.

In: Blood, Vol. 71, No. 1, 1988, p. 209-215.

Research output: Contribution to journalArticle

Hutt-Taylor, S. R. ; Harnish, D. ; Richardson, M. ; Ishizaka, T. ; Denburg, J. A. / Sodium butyrate and a T lymphocyte cell line-derived differentiation factor induce basophilic differentiation of the human promyelocytic leukemia cell line HL-60. In: Blood. 1988 ; Vol. 71, No. 1. pp. 209-215.
@article{7c0fd33f28b2416eada9968781aa9aa5,
title = "Sodium butyrate and a T lymphocyte cell line-derived differentiation factor induce basophilic differentiation of the human promyelocytic leukemia cell line HL-60",
abstract = "Sodium butyrate induces basophilic differentiation of HL-60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HL-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staining azurophilic with May-Grunwald-Giemsa and metachromatically with toluidine blue. The histamine content of induced HL-60 cells is 50 ng/106 cells with sodium butyrate alone or 190 ng/106 cells with butyrate in combination with Mo-CM. Induced cells release histamine in response to anti-IgE and have receptors for the Fc portion of human IgE. The basophilic cell-differentiating activity present in Mo-CM appears to be distinct from several other cytokines including recombinant human interleukin-1α, interleukin-2, interferon-γ, interferon-α, murine interleukin-3, erythroid-potentiating activity, and purified human granulocyte/macrophage colony-stimulating factor. This is the first demonstration of a cell line that is capable of differentiation along the basophil lineage and could provide a useful model for examining biochemical and molecular events associated with basophil differentiation.",
author = "Hutt-Taylor, {S. R.} and D. Harnish and M. Richardson and T. Ishizaka and Denburg, {J. A.}",
year = "1988",
language = "English (US)",
volume = "71",
pages = "209--215",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Sodium butyrate and a T lymphocyte cell line-derived differentiation factor induce basophilic differentiation of the human promyelocytic leukemia cell line HL-60

AU - Hutt-Taylor, S. R.

AU - Harnish, D.

AU - Richardson, M.

AU - Ishizaka, T.

AU - Denburg, J. A.

PY - 1988

Y1 - 1988

N2 - Sodium butyrate induces basophilic differentiation of HL-60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HL-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staining azurophilic with May-Grunwald-Giemsa and metachromatically with toluidine blue. The histamine content of induced HL-60 cells is 50 ng/106 cells with sodium butyrate alone or 190 ng/106 cells with butyrate in combination with Mo-CM. Induced cells release histamine in response to anti-IgE and have receptors for the Fc portion of human IgE. The basophilic cell-differentiating activity present in Mo-CM appears to be distinct from several other cytokines including recombinant human interleukin-1α, interleukin-2, interferon-γ, interferon-α, murine interleukin-3, erythroid-potentiating activity, and purified human granulocyte/macrophage colony-stimulating factor. This is the first demonstration of a cell line that is capable of differentiation along the basophil lineage and could provide a useful model for examining biochemical and molecular events associated with basophil differentiation.

AB - Sodium butyrate induces basophilic differentiation of HL-60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HL-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staining azurophilic with May-Grunwald-Giemsa and metachromatically with toluidine blue. The histamine content of induced HL-60 cells is 50 ng/106 cells with sodium butyrate alone or 190 ng/106 cells with butyrate in combination with Mo-CM. Induced cells release histamine in response to anti-IgE and have receptors for the Fc portion of human IgE. The basophilic cell-differentiating activity present in Mo-CM appears to be distinct from several other cytokines including recombinant human interleukin-1α, interleukin-2, interferon-γ, interferon-α, murine interleukin-3, erythroid-potentiating activity, and purified human granulocyte/macrophage colony-stimulating factor. This is the first demonstration of a cell line that is capable of differentiation along the basophil lineage and could provide a useful model for examining biochemical and molecular events associated with basophil differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0023838795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023838795&partnerID=8YFLogxK

M3 - Article

C2 - 2446679

AN - SCOPUS:0023838795

VL - 71

SP - 209

EP - 215

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -