SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model

Maged Harraz, Jennifer J. Marden, Weihong Zhou, Yulong Zhang, Aislinn Williams, Victor S. Sharov, Kathryn Nelson, Meihui Luo, Henry Paulson, Christian Schöneich, John F. Engelhardt

Research output: Contribution to journalArticle

Abstract

Neurodegeneration in familial amyotrophic lateral sclerosis (ALS) is associated with enhanced redox stress caused by dominant mutations in superoxide dismutase-1 (SOD1). SOD1 is a cytosolic enzyme that facilitates the conversion of superoxide (O2•-) to H2O2. Here we demonstrate that SOD1 is not just a catabolic enzyme, but can also directly regulate NADPH oxidase-dependent (Nox-dependent) O2 •- production by binding Rac1 and inhibiting its GTPase activity. Oxidation of Rac1 by H2O2 uncoupled SOD1 binding in a reversible fashion, producing a self-regulating redox sensor for Nox-derived O2•- production. This process of redox-sensitive uncoupling of SOD1 from Rac1 was defective in SOD1 ALS mutants, leading to enhanced Rac1/Nox activation in transgenic mouse tissues and cell lines expressing ALS SOD1 mutants. Glial cell toxicity associated with expression of SOD1 mutants in culture was significantly attenuated by treatment with the Nox inhibitor apocynin. Treatment of ALS mice with apocynin also significantly increased their average life span. This redox sensor mechanism may explain the gain-of-function seen with certain SOD1 mutations associated with ALS and defines new therapeutic targets.

Original languageEnglish (US)
Pages (from-to)659-670
Number of pages12
JournalJournal of Clinical Investigation
Volume118
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

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NADPH Oxidase
Oxidation-Reduction
Mutation
Amyotrophic Lateral Sclerosis
Amyotrophic lateral sclerosis 1
Superoxide Dismutase-1
GTP Phosphohydrolases
Enzymes
Neuroglia
Superoxides
Transgenic Mice
Cell Line

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Harraz, M., Marden, J. J., Zhou, W., Zhang, Y., Williams, A., Sharov, V. S., ... Engelhardt, J. F. (2008). SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. Journal of Clinical Investigation, 118(2), 659-670. https://doi.org/10.1172/JCI34060

SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. / Harraz, Maged; Marden, Jennifer J.; Zhou, Weihong; Zhang, Yulong; Williams, Aislinn; Sharov, Victor S.; Nelson, Kathryn; Luo, Meihui; Paulson, Henry; Schöneich, Christian; Engelhardt, John F.

In: Journal of Clinical Investigation, Vol. 118, No. 2, 01.02.2008, p. 659-670.

Research output: Contribution to journalArticle

Harraz, M, Marden, JJ, Zhou, W, Zhang, Y, Williams, A, Sharov, VS, Nelson, K, Luo, M, Paulson, H, Schöneich, C & Engelhardt, JF 2008, 'SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model', Journal of Clinical Investigation, vol. 118, no. 2, pp. 659-670. https://doi.org/10.1172/JCI34060
Harraz, Maged ; Marden, Jennifer J. ; Zhou, Weihong ; Zhang, Yulong ; Williams, Aislinn ; Sharov, Victor S. ; Nelson, Kathryn ; Luo, Meihui ; Paulson, Henry ; Schöneich, Christian ; Engelhardt, John F. / SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. In: Journal of Clinical Investigation. 2008 ; Vol. 118, No. 2. pp. 659-670.
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