Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish

Kyeong Won Yoo, Eun Hye Kim, Seung Hyun Jung, Myungchull Rhee, Bon Kyoung Koo, Ki Jun Yoon, Young Yun Kong, Cheol Hee Kim

Research output: Contribution to journalArticle

Abstract

Notch signaling has an evolutionarily conserved function for cell fate determination and stem cell maintenance. Previously, we identified a novel component of the Notch signaling pathway in zebrafish, mind bomb, which encodes an E3 ubiquitin ligase essential for Notch signal activation. Further studies showed that Mind bomb-/- mouse embryos exhibited pan-Notch phenotypes in various tissues, suggesting that Mind bomb function is conserved in mammals. Therefore we sought to understand the various molecular partners of Mind bomb using yeast two-hybrid screening. In this search we identified Sorting nexin 5 (Snx5) as a novel interacting partner of Mind bomb. Furthermore we demonstrated that Snx5 colocalizes with Mind bomb in early endosomal compartments, suggesting that Snx5 is important for Mind bomb trafficking. In addition, we identified zebrafish orthologue of Snx5 and showed that snx5 is predominantly expressed in hematopoietic and endothelial precursor cells in zebrafish. We also found defects in hematopoiesis and blood vessel development in snx5 morpholino-injected embryos. Taken together, we show that Snx5, a novel interacting partner of Mind bomb, may have an essential role for cell fate determination in early development.

Original languageEnglish (US)
Pages (from-to)4409-4416
Number of pages8
JournalFEBS Letters
Volume580
Issue number18
DOIs
Publication statusPublished - Aug 7 2006
Externally publishedYes

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Keywords

  • Endothelial precursor cells
  • Hematopoietic precursor cells
  • Mind bomb
  • Notch signaling
  • Sorting nexin 5
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yoo, K. W., Kim, E. H., Jung, S. H., Rhee, M., Koo, B. K., Yoon, K. J., ... Kim, C. H. (2006). Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish. FEBS Letters, 580(18), 4409-4416. https://doi.org/10.1016/j.febslet.2006.07.009