Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish

Kyeong Won Yoo, Eun Hye Kim, Seung Hyun Jung, Myungchull Rhee, Bon Kyoung Koo, Ki Jun Yoon, Young Yun Kong, Cheol Hee Kim

Research output: Contribution to journalArticle

Abstract

Notch signaling has an evolutionarily conserved function for cell fate determination and stem cell maintenance. Previously, we identified a novel component of the Notch signaling pathway in zebrafish, mind bomb, which encodes an E3 ubiquitin ligase essential for Notch signal activation. Further studies showed that Mind bomb-/- mouse embryos exhibited pan-Notch phenotypes in various tissues, suggesting that Mind bomb function is conserved in mammals. Therefore we sought to understand the various molecular partners of Mind bomb using yeast two-hybrid screening. In this search we identified Sorting nexin 5 (Snx5) as a novel interacting partner of Mind bomb. Furthermore we demonstrated that Snx5 colocalizes with Mind bomb in early endosomal compartments, suggesting that Snx5 is important for Mind bomb trafficking. In addition, we identified zebrafish orthologue of Snx5 and showed that snx5 is predominantly expressed in hematopoietic and endothelial precursor cells in zebrafish. We also found defects in hematopoiesis and blood vessel development in snx5 morpholino-injected embryos. Taken together, we show that Snx5, a novel interacting partner of Mind bomb, may have an essential role for cell fate determination in early development.

Original languageEnglish (US)
Pages (from-to)4409-4416
Number of pages8
JournalFEBS Letters
Volume580
Issue number18
DOIs
StatePublished - Aug 7 2006
Externally publishedYes

Fingerprint

Sorting Nexins
Bombs
Zebrafish
Carrier Proteins
Endothelial Cells
Morpholinos
Mammals
Ubiquitin-Protein Ligases
Blood vessels
Stem cells
Yeast
Screening
Embryonic Structures
Chemical activation
Tissue
Defects
Hematopoiesis
Blood Vessels
Stem Cells
Yeasts

Keywords

  • Endothelial precursor cells
  • Hematopoietic precursor cells
  • Mind bomb
  • Notch signaling
  • Sorting nexin 5
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yoo, K. W., Kim, E. H., Jung, S. H., Rhee, M., Koo, B. K., Yoon, K. J., ... Kim, C. H. (2006). Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish. FEBS Letters, 580(18), 4409-4416. https://doi.org/10.1016/j.febslet.2006.07.009

Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish. / Yoo, Kyeong Won; Kim, Eun Hye; Jung, Seung Hyun; Rhee, Myungchull; Koo, Bon Kyoung; Yoon, Ki Jun; Kong, Young Yun; Kim, Cheol Hee.

In: FEBS Letters, Vol. 580, No. 18, 07.08.2006, p. 4409-4416.

Research output: Contribution to journalArticle

Yoo, KW, Kim, EH, Jung, SH, Rhee, M, Koo, BK, Yoon, KJ, Kong, YY & Kim, CH 2006, 'Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish', FEBS Letters, vol. 580, no. 18, pp. 4409-4416. https://doi.org/10.1016/j.febslet.2006.07.009
Yoo, Kyeong Won ; Kim, Eun Hye ; Jung, Seung Hyun ; Rhee, Myungchull ; Koo, Bon Kyoung ; Yoon, Ki Jun ; Kong, Young Yun ; Kim, Cheol Hee. / Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish. In: FEBS Letters. 2006 ; Vol. 580, No. 18. pp. 4409-4416.
@article{ea8ad2656660448eb968bfdb20a7d810,
title = "Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish",
abstract = "Notch signaling has an evolutionarily conserved function for cell fate determination and stem cell maintenance. Previously, we identified a novel component of the Notch signaling pathway in zebrafish, mind bomb, which encodes an E3 ubiquitin ligase essential for Notch signal activation. Further studies showed that Mind bomb-/- mouse embryos exhibited pan-Notch phenotypes in various tissues, suggesting that Mind bomb function is conserved in mammals. Therefore we sought to understand the various molecular partners of Mind bomb using yeast two-hybrid screening. In this search we identified Sorting nexin 5 (Snx5) as a novel interacting partner of Mind bomb. Furthermore we demonstrated that Snx5 colocalizes with Mind bomb in early endosomal compartments, suggesting that Snx5 is important for Mind bomb trafficking. In addition, we identified zebrafish orthologue of Snx5 and showed that snx5 is predominantly expressed in hematopoietic and endothelial precursor cells in zebrafish. We also found defects in hematopoiesis and blood vessel development in snx5 morpholino-injected embryos. Taken together, we show that Snx5, a novel interacting partner of Mind bomb, may have an essential role for cell fate determination in early development.",
keywords = "Endothelial precursor cells, Hematopoietic precursor cells, Mind bomb, Notch signaling, Sorting nexin 5, Zebrafish",
author = "Yoo, {Kyeong Won} and Kim, {Eun Hye} and Jung, {Seung Hyun} and Myungchull Rhee and Koo, {Bon Kyoung} and Yoon, {Ki Jun} and Kong, {Young Yun} and Kim, {Cheol Hee}",
year = "2006",
month = "8",
day = "7",
doi = "10.1016/j.febslet.2006.07.009",
language = "English (US)",
volume = "580",
pages = "4409--4416",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "18",

}

TY - JOUR

T1 - Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish

AU - Yoo, Kyeong Won

AU - Kim, Eun Hye

AU - Jung, Seung Hyun

AU - Rhee, Myungchull

AU - Koo, Bon Kyoung

AU - Yoon, Ki Jun

AU - Kong, Young Yun

AU - Kim, Cheol Hee

PY - 2006/8/7

Y1 - 2006/8/7

N2 - Notch signaling has an evolutionarily conserved function for cell fate determination and stem cell maintenance. Previously, we identified a novel component of the Notch signaling pathway in zebrafish, mind bomb, which encodes an E3 ubiquitin ligase essential for Notch signal activation. Further studies showed that Mind bomb-/- mouse embryos exhibited pan-Notch phenotypes in various tissues, suggesting that Mind bomb function is conserved in mammals. Therefore we sought to understand the various molecular partners of Mind bomb using yeast two-hybrid screening. In this search we identified Sorting nexin 5 (Snx5) as a novel interacting partner of Mind bomb. Furthermore we demonstrated that Snx5 colocalizes with Mind bomb in early endosomal compartments, suggesting that Snx5 is important for Mind bomb trafficking. In addition, we identified zebrafish orthologue of Snx5 and showed that snx5 is predominantly expressed in hematopoietic and endothelial precursor cells in zebrafish. We also found defects in hematopoiesis and blood vessel development in snx5 morpholino-injected embryos. Taken together, we show that Snx5, a novel interacting partner of Mind bomb, may have an essential role for cell fate determination in early development.

AB - Notch signaling has an evolutionarily conserved function for cell fate determination and stem cell maintenance. Previously, we identified a novel component of the Notch signaling pathway in zebrafish, mind bomb, which encodes an E3 ubiquitin ligase essential for Notch signal activation. Further studies showed that Mind bomb-/- mouse embryos exhibited pan-Notch phenotypes in various tissues, suggesting that Mind bomb function is conserved in mammals. Therefore we sought to understand the various molecular partners of Mind bomb using yeast two-hybrid screening. In this search we identified Sorting nexin 5 (Snx5) as a novel interacting partner of Mind bomb. Furthermore we demonstrated that Snx5 colocalizes with Mind bomb in early endosomal compartments, suggesting that Snx5 is important for Mind bomb trafficking. In addition, we identified zebrafish orthologue of Snx5 and showed that snx5 is predominantly expressed in hematopoietic and endothelial precursor cells in zebrafish. We also found defects in hematopoiesis and blood vessel development in snx5 morpholino-injected embryos. Taken together, we show that Snx5, a novel interacting partner of Mind bomb, may have an essential role for cell fate determination in early development.

KW - Endothelial precursor cells

KW - Hematopoietic precursor cells

KW - Mind bomb

KW - Notch signaling

KW - Sorting nexin 5

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=33746347054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746347054&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.07.009

DO - 10.1016/j.febslet.2006.07.009

M3 - Article

C2 - 16857196

AN - SCOPUS:33746347054

VL - 580

SP - 4409

EP - 4416

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 18

ER -