Small subunit ribosomal RNA of Blastomyces dermatitidis: Sequence and phylogenetic analysis

A. Geber, D. E. Higgins, A. P. Waters, J. E. Bennett, T. F. McCutchan

Research output: Contribution to journalArticle

Abstract

We determined the small subunit (18S) ribosomal RNA sequence of the dimorphic fungus Blastomyces dermatitidis. The sequence was compared to that of fourteen other eukaryotic organisms, ten of which were higher fungi, and an evolutionary tree was constructed based on these sequences. B. dermatitidis aligned most closely with the Ascomycetes Neurospora crassa and Podospora anserina, in agreement with previous phylogenetic analysis based on morphological criteria. Phase-specific cDNA clones derived by reverse transcription of RNA isolated from the yeast and mycelial phases of B. dermatitidis were also sequenced. The 18S ribosome sequence was found to be the same in both phases. Heterogeneity was found at both the genomic and RNA level at position 1352.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalJournal of General Microbiology
Volume138
Issue number2
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Microbiology

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