Small-animal PET imaging of isolated perfused rat heart

Tomohiko Yamane, Min Jae Park, Dominik Richter, Stephan G. Nekolla, Mehrbod Som Som Javadi, Constantin Lapa, Samuel Samnick, Andreas K. Buck, Ken Herrmann, Takahiro Higuchi

Research output: Contribution to journalArticle

Abstract

The assessment of myocardial radiotracer kinetics, including cardiac extraction fraction and washout, requires the study of isolated perfused hearts to avoid analytic error due to tracer recirculation and systemic metabolites. Analysis of the isolated perfused rat heart by a high-resolution small-animal PET system may offer both reliable evaluation of cardiac tracer kinetics and tomographic images. Methods: An isolated perfused heart system was modified to accommodate the small PET gantry bore size. Isolated rat hearts were perfused via the Langendorff method under a constant flow of Krebs-Henseleit buffer containing 18F-FDG with a rate of 5 mL/min and placed in the field of view of the commercially available small-animal PET system. Dynamic PET imaging was then performed, with 18F-FDG uptake in the isolated perfused heart verified by g counter measurements. Additionally, a rat heart of myocardial infarction was also studied in this system with static PET imaging. Results: Dynamic PET acquisition of the isolated heart under constant 18F-FDG infusion demonstrated continuous increase of activity in the heart. Correlation between cardiac activity (MBq) measured with the PET system and measurements made with the g counter were excellent (R2 5 0.98, P > 0.001, n 5 10). Tracer input rate (MBq/min) was also well correlated with cardiac tracer uptake rate (MBq/min) (R2 5 0.87, P > 0.001, n 5 12). PET imaging of the heart with myocardial infarction showed a clear tracer uptake defect corresponding to the location of scar tissue identified by autoradiography and histology. Conclusion: Combining the Langendorff method of isolated rat heart perfusion with high-resolution small-animal PET allows for the reliable quantification of myocardial tracer kinetics. This novel assay is readily adapted to available small-animal PET systems and may be useful for understanding myocardial PET tracer kinetics.

Original languageEnglish (US)
Pages (from-to)495-499
Number of pages5
JournalJournal of Nuclear Medicine
Volume55
Issue number3
DOIs
StatePublished - Mar 1 2014

Fingerprint

Fluorodeoxyglucose F18
Myocardial Infarction
Autoradiography
Cicatrix
Histology
Perfusion

Keywords

  • Isolated perfused heart
  • Myocardial infarction
  • PET
  • Rat heart

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

Yamane, T., Park, M. J., Richter, D., Nekolla, S. G., Javadi, M. S. S., Lapa, C., ... Higuchi, T. (2014). Small-animal PET imaging of isolated perfused rat heart. Journal of Nuclear Medicine, 55(3), 495-499. https://doi.org/10.2967/jnumed.113.129429

Small-animal PET imaging of isolated perfused rat heart. / Yamane, Tomohiko; Park, Min Jae; Richter, Dominik; Nekolla, Stephan G.; Javadi, Mehrbod Som Som; Lapa, Constantin; Samnick, Samuel; Buck, Andreas K.; Herrmann, Ken; Higuchi, Takahiro.

In: Journal of Nuclear Medicine, Vol. 55, No. 3, 01.03.2014, p. 495-499.

Research output: Contribution to journalArticle

Yamane, T, Park, MJ, Richter, D, Nekolla, SG, Javadi, MSS, Lapa, C, Samnick, S, Buck, AK, Herrmann, K & Higuchi, T 2014, 'Small-animal PET imaging of isolated perfused rat heart', Journal of Nuclear Medicine, vol. 55, no. 3, pp. 495-499. https://doi.org/10.2967/jnumed.113.129429
Yamane T, Park MJ, Richter D, Nekolla SG, Javadi MSS, Lapa C et al. Small-animal PET imaging of isolated perfused rat heart. Journal of Nuclear Medicine. 2014 Mar 1;55(3):495-499. https://doi.org/10.2967/jnumed.113.129429
Yamane, Tomohiko ; Park, Min Jae ; Richter, Dominik ; Nekolla, Stephan G. ; Javadi, Mehrbod Som Som ; Lapa, Constantin ; Samnick, Samuel ; Buck, Andreas K. ; Herrmann, Ken ; Higuchi, Takahiro. / Small-animal PET imaging of isolated perfused rat heart. In: Journal of Nuclear Medicine. 2014 ; Vol. 55, No. 3. pp. 495-499.
@article{91fc839246da4eb98ad3fafc218015da,
title = "Small-animal PET imaging of isolated perfused rat heart",
abstract = "The assessment of myocardial radiotracer kinetics, including cardiac extraction fraction and washout, requires the study of isolated perfused hearts to avoid analytic error due to tracer recirculation and systemic metabolites. Analysis of the isolated perfused rat heart by a high-resolution small-animal PET system may offer both reliable evaluation of cardiac tracer kinetics and tomographic images. Methods: An isolated perfused heart system was modified to accommodate the small PET gantry bore size. Isolated rat hearts were perfused via the Langendorff method under a constant flow of Krebs-Henseleit buffer containing 18F-FDG with a rate of 5 mL/min and placed in the field of view of the commercially available small-animal PET system. Dynamic PET imaging was then performed, with 18F-FDG uptake in the isolated perfused heart verified by g counter measurements. Additionally, a rat heart of myocardial infarction was also studied in this system with static PET imaging. Results: Dynamic PET acquisition of the isolated heart under constant 18F-FDG infusion demonstrated continuous increase of activity in the heart. Correlation between cardiac activity (MBq) measured with the PET system and measurements made with the g counter were excellent (R2 5 0.98, P > 0.001, n 5 10). Tracer input rate (MBq/min) was also well correlated with cardiac tracer uptake rate (MBq/min) (R2 5 0.87, P > 0.001, n 5 12). PET imaging of the heart with myocardial infarction showed a clear tracer uptake defect corresponding to the location of scar tissue identified by autoradiography and histology. Conclusion: Combining the Langendorff method of isolated rat heart perfusion with high-resolution small-animal PET allows for the reliable quantification of myocardial tracer kinetics. This novel assay is readily adapted to available small-animal PET systems and may be useful for understanding myocardial PET tracer kinetics.",
keywords = "Isolated perfused heart, Myocardial infarction, PET, Rat heart",
author = "Tomohiko Yamane and Park, {Min Jae} and Dominik Richter and Nekolla, {Stephan G.} and Javadi, {Mehrbod Som Som} and Constantin Lapa and Samuel Samnick and Buck, {Andreas K.} and Ken Herrmann and Takahiro Higuchi",
year = "2014",
month = "3",
day = "1",
doi = "10.2967/jnumed.113.129429",
language = "English (US)",
volume = "55",
pages = "495--499",
journal = "Journal of Nuclear Medicine",
issn = "0161-5505",
publisher = "Society of Nuclear Medicine Inc.",
number = "3",

}

TY - JOUR

T1 - Small-animal PET imaging of isolated perfused rat heart

AU - Yamane, Tomohiko

AU - Park, Min Jae

AU - Richter, Dominik

AU - Nekolla, Stephan G.

AU - Javadi, Mehrbod Som Som

AU - Lapa, Constantin

AU - Samnick, Samuel

AU - Buck, Andreas K.

AU - Herrmann, Ken

AU - Higuchi, Takahiro

PY - 2014/3/1

Y1 - 2014/3/1

N2 - The assessment of myocardial radiotracer kinetics, including cardiac extraction fraction and washout, requires the study of isolated perfused hearts to avoid analytic error due to tracer recirculation and systemic metabolites. Analysis of the isolated perfused rat heart by a high-resolution small-animal PET system may offer both reliable evaluation of cardiac tracer kinetics and tomographic images. Methods: An isolated perfused heart system was modified to accommodate the small PET gantry bore size. Isolated rat hearts were perfused via the Langendorff method under a constant flow of Krebs-Henseleit buffer containing 18F-FDG with a rate of 5 mL/min and placed in the field of view of the commercially available small-animal PET system. Dynamic PET imaging was then performed, with 18F-FDG uptake in the isolated perfused heart verified by g counter measurements. Additionally, a rat heart of myocardial infarction was also studied in this system with static PET imaging. Results: Dynamic PET acquisition of the isolated heart under constant 18F-FDG infusion demonstrated continuous increase of activity in the heart. Correlation between cardiac activity (MBq) measured with the PET system and measurements made with the g counter were excellent (R2 5 0.98, P > 0.001, n 5 10). Tracer input rate (MBq/min) was also well correlated with cardiac tracer uptake rate (MBq/min) (R2 5 0.87, P > 0.001, n 5 12). PET imaging of the heart with myocardial infarction showed a clear tracer uptake defect corresponding to the location of scar tissue identified by autoradiography and histology. Conclusion: Combining the Langendorff method of isolated rat heart perfusion with high-resolution small-animal PET allows for the reliable quantification of myocardial tracer kinetics. This novel assay is readily adapted to available small-animal PET systems and may be useful for understanding myocardial PET tracer kinetics.

AB - The assessment of myocardial radiotracer kinetics, including cardiac extraction fraction and washout, requires the study of isolated perfused hearts to avoid analytic error due to tracer recirculation and systemic metabolites. Analysis of the isolated perfused rat heart by a high-resolution small-animal PET system may offer both reliable evaluation of cardiac tracer kinetics and tomographic images. Methods: An isolated perfused heart system was modified to accommodate the small PET gantry bore size. Isolated rat hearts were perfused via the Langendorff method under a constant flow of Krebs-Henseleit buffer containing 18F-FDG with a rate of 5 mL/min and placed in the field of view of the commercially available small-animal PET system. Dynamic PET imaging was then performed, with 18F-FDG uptake in the isolated perfused heart verified by g counter measurements. Additionally, a rat heart of myocardial infarction was also studied in this system with static PET imaging. Results: Dynamic PET acquisition of the isolated heart under constant 18F-FDG infusion demonstrated continuous increase of activity in the heart. Correlation between cardiac activity (MBq) measured with the PET system and measurements made with the g counter were excellent (R2 5 0.98, P > 0.001, n 5 10). Tracer input rate (MBq/min) was also well correlated with cardiac tracer uptake rate (MBq/min) (R2 5 0.87, P > 0.001, n 5 12). PET imaging of the heart with myocardial infarction showed a clear tracer uptake defect corresponding to the location of scar tissue identified by autoradiography and histology. Conclusion: Combining the Langendorff method of isolated rat heart perfusion with high-resolution small-animal PET allows for the reliable quantification of myocardial tracer kinetics. This novel assay is readily adapted to available small-animal PET systems and may be useful for understanding myocardial PET tracer kinetics.

KW - Isolated perfused heart

KW - Myocardial infarction

KW - PET

KW - Rat heart

UR - http://www.scopus.com/inward/record.url?scp=84899481884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899481884&partnerID=8YFLogxK

U2 - 10.2967/jnumed.113.129429

DO - 10.2967/jnumed.113.129429

M3 - Article

C2 - 24481025

AN - SCOPUS:84899481884

VL - 55

SP - 495

EP - 499

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

SN - 0161-5505

IS - 3

ER -